| Naslov: | Physiologically achievable concentration of 2-deoxy-D-glucose stimulates IFN-γ secretion in activated T cells in vitro |
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| Avtorji: | ID Repas, Jernej (Avtor)
ID Frlic, Tjaša (Avtor)
ID Snedec, Tadeja (Avtor)
ID Kopitar, Andreja Nataša (Avtor)
ID Sourij, Harald (Avtor)
ID Janež, Andrej (Avtor)
ID Pavlin, Mojca (Avtor) |
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| Datoteke: |  PDF - Predstavitvena datoteka, prenos (2,76 MB)
MD5: 19B42B5FE600868704B0564989C0F230
 URL - Izvorni URL, za dostop obiščite https://www.mdpi.com/1422-0067/25/19/10384
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  UKC LJ - Univerzitetni klinični center Ljubljana
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| Povzetek: | 2-deoxy-D-glucose (2DG) is a glycolysis and protein N-glycosylation inhibitor with promising anti-tumor and immunomodulatory effects. However, 2DG can also suppress T cell function, including IFN-γ secretion. Few human T cell studies have studied low-dose 2DG, which can increase IFN-γ in a Jurkat clone. We therefore investigated 2DG’s effect on IFN-γ in activated human T cells from PBMCs, with 2DG treatment commenced either concurrently with activation or 48 h after activation. Concurrent 2DG treatment decreased IFN-γ secretion in a dose-dependent manner. However, 2DG treatment of pre-activated T cells had a hormetic effect on IFN-γ, with 0.15–0.6 mM 2DG (achievable in vivo) increasing and >2.4 mM 2DG reducing its secretion. In contrast, IL-2 levels declined monotonously with increasing 2DG concentration. Lower 2DG concentrations reduced PD-1 and increased CD69 expression regardless of treatment timing. The absence of increased T-bet or Eomes expression or IFNG transcription suggests another downstream mechanism. 2DG dose-dependently induced the unfolded protein response, suggesting a possible role in increased IFN-γ secretion, possibly by increasing the ER folding capacity for IFN-γ via increased chaperone expression. Overall, low-dose, short-term 2DG exposure could potentially improve the T cell anti-tumor response. |
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| Ključne besede: | T cells, interferon gamma, 2-deoxy-D-glucose |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Leto izida: | 2024 |
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| Št. strani: | str. 1-21 |
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| Številčenje: | Vol. 25, iss. 19 , [article no.] 10384 |
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| PID: | 20.500.12556/DiRROS-29992  |
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| UDK: | 616.379 |
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| ISSN pri članku: | 1422-0067 |
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| DOI: | 10.3390/ijms251910384 |
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| COBISS.SI-ID: | 208868867 |
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| Opomba: | Nasl. z nasl. zaslona;
Opis vira z dne 30. 9. 2024;
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| Datum objave v DiRROS: | 11.06.2026 |
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| Število ogledov: | 203 |
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| Število prenosov: | 185 |
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| Metapodatki: |    |
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