| Naslov: | Optimised extraction and HPLC–DAD determination of steroidal glycoalkaloids in potato tubers : analytical validation and environmental sustainability evaluation |
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| Avtorji: | ID Ben Ammar, Hajer (Avtor)
ID Dolničar, Peter (Avtor)
ID Pipan, Barbara (Avtor)
ID Sinkovič, Lovro (Avtor) |
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| Datoteke: |  URL - Izvorni URL, za dostop obiščite https://www.sciencedirect.com/science/article/pii/S0021967326004942?via%3Dihub
 PDF - Predstavitvena datoteka, prenos (6,47 MB)
MD5: 3DD23369587B8174FBA374C4E3AD50C6
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  KIS - Kmetijski inštitut Slovenije
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| Povzetek: | Steroidal glycoalkaloids ( α-solanine and α-chaconine) are major potato toxins whose accurate quantification is often challenged by matrix effects and chromatographic selectivity. This study reports the optimisation and validation of a robust reversed-phase HPLC-DAD method for the determination of glycoalkaloids in potato tissues, combined with a systematic assessment of the environmental sustainability of the analytical workflow. Extraction conditions were optimized by integrating systematic solvent screening with a factorial experimental design to evaluate the effect of key operational variables on glycoalkaloid recovery and refine extraction performance The resulting extraction medium improved extract cleanliness and chromatographic stability. A subsequent C18 solid-phase extraction SPE step further reduced matrix effect and enhanced analytical selectivity. Chromatographic separation was achieved on a conventional C18 column using an acetonitrile-water gradient, providing baseline resolution (Rs > 1.5) of α 2 -solanine and α-chaconine with stable diode-array detection (DAD) at 205 nm. The method was validated according to ICH Q2(R2) and AOAC guidelines, demonstrating excellent linearity (R > 0.995), low limits of detection (0.462–0.767 µg/mL) and quantification (1.540–2.558 µg/mL), high precision (≤ 5% RSD), and recoveries of 90–105%. Robustness testing confirmed minimal sensitivity to routine variations in chromatographic parameters. Application to potato breeding lines demonstrated reliable quantification and revealed genotype-dependent variation in glycoalkaloid content. The environmental assessment of the analytical procedure was evaluated using Analytical Eco-scale, GAPI and AGREE metrics, demonstrating reduced solvent consumption, avoidance of strongly acidic extraction conditions, and a simplified sample preparation workflow. The proposed HPLC-DAD workflow provides a validated, high-performance, and environmentally conscious approach for routine GA quantification, suitable for food-safety monitoring, regulatory compliance and breeding programme applications. |
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| Ključne besede: | α-chaconine, α-solanine, analytical method, HPLC-DAD, potato tubers, environmental sustainability |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 03.06.2026 |
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| Leto izida: | 2026 |
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| Št. strani: | str. 1-18 |
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| Številčenje: | Vol. 1783, [article no.] 467165 |
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| PID: | 20.500.12556/DiRROS-29950  |
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| UDK: | 633 |
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| ISSN pri članku: | 1873-3778 |
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| DOI: | 10.1016/j.chroma.2026.467165 |
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| COBISS.SI-ID: | 280684547 |
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| Opomba: | Soavtorji: Peter Dolničar, Barbara Pipan, Lovro Sinkovič;
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| Datum objave v DiRROS: | 10.06.2026 |
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| Število ogledov: | 45 |
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| Število prenosov: | 39 |
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| Metapodatki: |    |
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