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Naslov:An international comparability study on quantification of mRNA gene expression ratios: CCQM-P103.1
Avtorji:ID Devonshire, Alison S. (Avtor)
ID Demšar, Tina (Avtor)
ID Žel, Jana (Avtor)
ID Blejec, Andrej (Avtor)
ID Milavec, Mojca (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (1,78 MB)
MD5: 64D199C43AC0EB7DF78AD7D3CA19BA15
 
URL URL - Izvorni URL, za dostop obiščite https://doi.org/10.1016/j.bdq.2016.05.003
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Measurement of RNA can be used to study and monitor a range of infectious and non-communicable diseases, with profiling of multiple gene expression mRNA transcripts being increasingly applied to cancer stratification and prognosis. An international comparison study (Consultative Committee for Amount of Substance (CCQM)-P103.1) was performed in order to evaluate the comparability of measurements of RNA copy number ratio for multiple gene targets between two samples. Six exogenous synthetic targets comprising of External RNA Control Consortium (ERCC) standards were measured alongside transcripts for three endogenous gene targets present in the background of human cell line RNA. The study was carried out under the auspices of the Nucleic Acids (formerly Bioanalysis) Working Group of the CCQM. It was coordinated by LGC (United Kingdom) with the support of National Institute of Standards and Technology (USA) and results were submitted from thirteen National Metrology Institutes and Designated Institutes. The majority of laboratories performed RNA measurements using RT-qPCR, with datasets also being submitted by two laboratories based on reverse transcription digital polymerase chain reaction and one laboratory using a next-generation sequencing method. In RT-qPCR analysis, the RNA copy number ratios between the two samples were quantified using either a standard curve or a relative quantification approach. In general, good agreement was observed between the reported results of ERCC RNA copy number ratio measurements. Measurements of the RNA copy number ratios for endogenous genes between the two samples were also consistent between the majority of laboratories. Some differences in the reported values and confidence intervals (‘measurement uncertainties’) were noted which may be attributable to choice of measurement method or quantification approach. This highlights the need for standardised practices for the calculation of fold change ratios and uncertainties in the area of gene expression profiling.
Ključne besede:RNA copy number ratio, RT-qPCR, gene expression, normalisation, standardisation, molecular diagnostic, transcriptomics, cancer, diagnostics, biomarker identification and validation
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:01.06.2016
Leto izida:2016
Št. strani:str. 15-28
Številčenje:Vol. 8
PID:20.500.12556/DiRROS-21601 Novo okno
UDK:577.2
ISSN pri članku:Y507-7052
DOI:10.1016/j.bdq.2016.05.003 Novo okno
COBISS.SI-ID:3906639 Novo okno
Opomba:Nasl. z nasl. zaslona; Opis vira z dne 9. 6. 2016;
Datum objave v DiRROS:04.03.2025
Število ogledov:125
Število prenosov:53
Metapodatki:XML DC-XML DC-RDF
:
DEVONSHIRE, Alison S., DEMŠAR, Tina, ŽEL, Jana, BLEJEC, Andrej in MILAVEC, Mojca, 2016, An international comparability study on quantification of mRNA gene expression ratios: CCQM-P103.1. Biomolecular detection and quantification [na spletu]. 2016. Vol. 8, p. 15–28. [Dostopano 9 april 2025]. DOI 10.1016/j.bdq.2016.05.003. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=21601
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Gradivo je del revije

Naslov:Biomolecular detection and quantification
Založnik:Elsevier
COBISS.SI-ID:3411023 Novo okno

Gradivo je financirano iz projekta

Financer:RCUK - Research Council UK
Program financ.:National Measurement System

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Licenca:CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
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