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Naslov:Grapevine flavescence dorée (FD) follow up Vitisens, GRAFDEPI and Qdetect (GRAFDEPI2) : final report
Avtorji:ID Dermastia, Marina (Avtor)
ID Reisenzein, Helga (Avtor)
ID Ferretti, Luca (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (1,16 MB)
MD5: E957FDFF891641A9C367E9477E086EB0
 
URL URL - Izvorni URL, za dostop obiščite https://www.euphresco.net/media/project_reports/grafdepi2_final_report.pdf
 
Jezik:Angleški jezik
Tipologija:2.12 - Končno poročilo o rezultatih raziskav
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Europe is the world’s main producer and exporter of grapevine planting material and wine. This important economic sector is facing epidemic threats of at least 10 grapevine yellows diseases (GY) caused by phytoplasmas. In Europe the main phytoplasmas associated with GY are ‘Candidatus’ phytoplasma solani’ (BNp), which is a causal agent of bois noir and FDp, which causes flavescence dorée. Phytoplasmas are notoriously difficult to detect and identify and their specific detection relies exclusively on the molecular methods. Recently new methods, which are reliable, sensitive, fast, less expensive and suitable for using onsites, have been introduced. Among them is a loop-mediated isothermal amplification (LAMP) method, with several advantages (e.g. low sensitivity to plant extracts inhibitors, speed, robustness, simplicity of use) over the other methods (e.g. the real-time PCR). In a recently finished FP7 project VITISENS, a new LAMP protocols have been developed for specific detection of FDp, however, they have not been tested in the interlaboratories trials. In addition, there is no validated LAMP protocol available for the specific detection of BNp at the moment. The main objectives of this project were: (1) Development of new loop-mediated isothermal amplification (LAMP) based protocols for accurate, reliable, fast and affordable diagnostics of ‘Candidatus Phytoplasma solani’ (BNp), which will be applicable in-field (2) To study new possible hosts plants and insect vectors of phytoplasma FDp. (3) To organize an interlaboratory test performance study (TPS) to obtain validation parameters for the selected LAMP protocols for BNp, as well as for the LAMP assay for FDp detection developed in the course of the FP7 project VITISENS.
Ključne besede:phytoplasmas, grapevine yellows diseases, LAMP, real-time PCR
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:11.11.2015
Kraj izida:[S. l.]
Kraj izvedbe:[S. l.]
Založnik:EPPO
Leto izida:2015
Leto izvedbe:2015
Št. strani:35, XLVI str.
PID:20.500.12556/DiRROS-20425 Novo okno
UDK:632
COBISS.SI-ID:3812175 Novo okno
Datum objave v DiRROS:16.09.2024
Število ogledov:158
Število prenosov:72
Metapodatki:XML DC-XML DC-RDF
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Gradivo je financirano iz projekta

Financer:Drugi - Drug financer ali več financerjev
Program financ.:EUPHRESCO
Naslov:Grapevine flavescence dorée (FD) follow up Vitisens, GRAFDEPI and Qdetect
Akronim:GRAFDEPI2

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