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Naslov:The efficient method for simultaneous monitoring of the culturable as well as nonculturable airborne microorganisms
Avtorji:ID Hubad, Barbara (Avtor)
ID Lapanje, Aleš (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (436,95 KB)
MD5: 88B4E2499807F7F20BA1115A0E27350D
 
URL URL - Izvorni URL, za dostop obiščite https://doi.org/10.1371/journal.pone.0082186
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Cultivation-based microbiological methods are a gold standard for monitoring of airborne micro-organisms to determine the occupational exposure levels or transmission paths of a particular infectious agent. Some highly contagious microorganisms are not easily culturable but it is becoming evident that cultivation and molecular methods are complementary and in these cases highly relevant. We report a simple and efficient method for sampling and analyzing airborne bacteria with an impactor-type high-flow-rate portable air sampler, currently used for monitoring culturable bacteria or fungi. A method is reported for extraction of nucleic acids from impacted cells without prior cultivation and using agarose as a sampling matrix. The DNA extraction efficiency was determined in spiked samples and, samples taken from a wastewater treatment plant and an alpine area. The abundance, diversity and quantity of total bacteria were analysed by a quantitative polymerase chain reaction, and by construction and analysis of clone libraries. The method does not interfere with downstream PCR analysis and can cover the gap between traditional culture and molecular techniques of bioaerosol monitoring.
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:20.12.2013
Leto izida:2013
Št. strani:Str. 1-9; e82186
Številčenje:Vol. 8, št. 12
PID:20.500.12556/DiRROS-20024 Novo okno
UDK:579.6
ISSN pri članku:1932-6203
DOI:10.1371/journal.pone.0082186 Novo okno
COBISS.SI-ID:3029583 Novo okno
Opomba:avtorski PDF; e82186;
Datum objave v DiRROS:02.08.2024
Število ogledov:481
Število prenosov:218
Metapodatki:XML DC-XML DC-RDF
:
HUBAD, Barbara in LAPANJE, Aleš, 2013, The efficient method for simultaneous monitoring of the culturable as well as nonculturable airborne microorganisms. PloS one [na spletu]. 2013. Vol. 8, no. 12, p. 1–9; e82186. [Dostopano 4 april 2025]. DOI 10.1371/journal.pone.0082186. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=20024
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Gradivo je del revije

Naslov:PloS one
Založnik:Public Library of Science
ISSN:1932-6203
COBISS.SI-ID:2005896 Novo okno

Gradivo je financirano iz projekta

Financer:EC - European Commission
Številka projekta:261810
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Akronim:MULTISENSE CHIP
Financer:EC - European Commission
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Naslov:Integrated Biotechnological Solutions for Combating Marine Oil Spills
Akronim:KILL●SPILL
Financer:Drugi - Drug financer ali več financerjev
Številka projekta:P-MR-07/55
Financer:EC - European Commission
Program financ.:the European Commission Seventh Framework program
Številka projekta:A-1087-RT-GC
Naslov:Patch

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

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