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Naslov:Filling the gaps in diagnostics of Pepino mosaic virus and Potato spindle tuber viroid in water and tomato seeds and leaves
Avtorji:ID Mehle, Nataša (Avtor)
ID Kogovšek, Polona (Avtor)
ID Rački, Nejc (Avtor)
ID Jakomin, Tjaša (Avtor)
ID Gutiérrez-Aguirre, Ion (Avtor)
ID Kramberger, Petra (Avtor)
ID Ravnikar, Maja (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (312,20 KB)
MD5: 6730A5CAAC1EFBF75A9F9A918923FCB1
 
URL URL - Izvorni URL, za dostop obiščite https://doi.org/10.1111/ppa.12710
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Waterborne and seedborne Pepino mosaic virus (PepMV) and Potato spindle tuber viroid (PSTVd) pose serious threats to tomato production due to seed transmission and mechanical transmission, coupled with their long-term stability outside the host plant. Therefore, rapid and sensitive diagnostic procedures are needed to prevent the spread of these quarantine pathogens. In particular, water and seed contamination are difficult to detect and confirm without efficient concentration methods. This study presents procedures that improve detection of PSTVd from tomato seeds and leaf tissue, and PepMV from water and tomato leaf tissue. For efficient concentration of PepMV from water samples, a procedure was optimized using convective interaction media monolithic chromatography columns, which provides concentration by three orders of magnitude. For concentration of PSTVd from seed extracts, an easy-to-use and efficient method was developed based on RNA binding to positively charged anion-exchange resin beads that provides up to 100-fold more sensitive detection in comparison with procedures without a concentration step. This thus allows confirmation of RT-qPCR results with sequencing of RT-PCR products in samples with low viroid levels. In addition, reverse-transcription loop-mediated isothermal amplification assays for detection of PSTVd and PepMV were optimized and adapted to both laboratory and on-site testing requirements. This allows rapid detection of these pathogens in crude leaf homogenates, in under 30 min. These procedures of concentration and detection are shown to be efficient and to fill the gaps in diagnostics of PepMV and PSTVd, especially when these pathogens are present at low levels in difficult matrices such as water and seeds.
Ključne besede:PSTVd, PepMV, seeds, water, concentration, loop-mediated isothermal amplification
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:01.09.2017
Leto izida:2017
Št. strani:str. 1191-1201
Številčenje:Vol. 66, issue 7
PID:20.500.12556/DiRROS-19712 Novo okno
UDK:577.2
ISSN pri članku:0032-0862
DOI:10.1111/ppa.12710 Novo okno
COBISS.SI-ID:4302159 Novo okno
Datum objave v DiRROS:24.07.2024
Število ogledov:542
Število prenosov:245
Metapodatki:XML DC-XML DC-RDF
:
MEHLE, Nataša, KOGOVŠEK, Polona, RAČKI, Nejc, JAKOMIN, Tjaša, GUTIÉRREZ-AGUIRRE, Ion, KRAMBERGER, Petra in RAVNIKAR, Maja, 2017, Filling the gaps in diagnostics of Pepino mosaic virus and Potato spindle tuber viroid in water and tomato seeds and leaves. Plant Pathology [na spletu]. 2017. Vol. 66, no. 7, p. 1191–1201. [Dostopano 3 april 2025]. DOI 10.1111/ppa.12710. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=19712
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Gradivo je del revije

Naslov:Plant Pathology
Skrajšan naslov:Plant Pathol.
Založnik:Her Majesty's Stationery Office
ISSN:0032-0862
COBISS.SI-ID:5661191 Novo okno

Gradivo je financirano iz projekta

Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:L4-5525-2013
Naslov:Študij epidemiologije in raznolikosti mikrobnih povzročiteljev bolezni rastlin
Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:P4-0165-2015
Naslov:Biotehnologija in sistemska biologija rastlin
Financer:EC - European Commission
Program financ.:ArimNet
Naslov:Emergent viruses and virus vectors in Mediterranean Basin crops
Akronim:Emaramb

Licence

Licenca:CC BY-NC 4.0, Creative Commons Priznanje avtorstva-Nekomercialno 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by-nc/4.0/deed.sl
Opis:Licenca Creative Commons, ki prepoveduje komercialno uporabo, vendar uporabniki ne rabijo upravljati materialnih avtorskih pravic na izpeljanih delih z enako licenco.

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