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Naslov:Identification of proteins associated with clinical and pathological features of proliferative diabetic retinopathy in vitreous and fibrovascular membranes
Avtorji:ID Klaassen, Ingeborg (Avtor)
ID Vries, Ewout W. de (Avtor)
ID Vogels, Ilse M.C. (Avtor)
ID Kampen, Antoine H. C. van (Avtor)
ID Bosscha, Machteld I. (Avtor)
ID Steel, David H. W. (Avtor)
ID Noorden, Cornelis J. F. van (Avtor)
ID Lesnik-Oberstein, Sarit Y (Avtor)
ID Schlingemann, Reinier O. (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0187304
 
.pdf PDF - Predstavitvena datoteka, prenos (1,42 MB)
MD5: E42E8441278944777DC60B9059D3A550
 
URL URL - Izvorni URL, za dostop obiščite https://doi.org/10.1371/journal.pone.0187304
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Purpose To identify the protein profiles in vitreous associated with retinal fibrosis, angiogenesis, and neurite formation in epiretinal fibrovascular membranes (FVMs) in patients with proliferative diabetic retinopathy (PDR). Methods Vitreous samples of 5 non-diabetic control patients with vitreous debris and 7 patients with PDR membranes were screened for 507 preselected proteins using the semi-quantitative RayBio® L-series 507 antibody array. From this array, 60 proteins were selected for a custom quantitative antibody array (Raybiotech, Human Quantibody® array), analyzing 7 control patients, 8 PDR patients with FVMs, and 5 PDR patients without FVMs. Additionally, mRNA levels of proteins of interest were measured in 10 PDR membranes and 11 idiopathic membranes and in retinal tissues and cells to identify possible sources of protein production. Results Of the 507 proteins screened, 21 were found to be significantly elevated in PDR patients, including neurogenic and angiogenic factors such as neuregulin 1 (NRG1), nerve growth factor receptor (NGFR), placental growth factor (PlGF) and platelet derived growth factor (PDGF). Angiopoietin-2 (Ang2) concentrations were strongly correlated to the degree of fibrosis and the presence of FVMs in patients with PDR. Protein correlation analysis showed PDGF to be extensively co-regulated with other proteins, including thrombospondin-1 and Ang2. mRNA levels of glial-derived and brain/derived neurotrophic factor (GDNF and BDNF) were elevated in PDR membranes. These results were validated in a second study of 52 vitreous samples of 32 PDR patients and 20 control patients. Conclusions This exploratory study reveals protein networks that potentially contribute to neurite outgrowth, angiogenesis and fibrosis in the formation of fibrovascular membranes in PDR. We identified a possible role of Ang2 in fibrosis and the formation of FVMs, and of the neurotrophic factors NRG1, PDGF and GDNF in neurite growth that occurs in all FVMs in PDR.
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:02.11.2017
Leto izida:2017
Št. strani:str. 1-21
Številčenje:Vol. 12, iss. 11, e0187304
PID:20.500.12556/DiRROS-19703 Novo okno
UDK:577
ISSN pri članku:1932-6203
DOI:10.1371/journal.pone.0187304 Novo okno
COBISS.SI-ID:40482309 Novo okno
Opomba:Nasl. z nasl. zaslona; Opis vira z dne 17. 3. 2020;
Datum objave v DiRROS:25.07.2024
Število ogledov:422
Število prenosov:351
Metapodatki:XML DC-XML DC-RDF
:
KLAASSEN, Ingeborg, VRIES, Ewout W. de, VOGELS, Ilse M.C., KAMPEN, Antoine H. C. van, BOSSCHA, Machteld I., STEEL, David H. W., NOORDEN, Cornelis J. F. van, LESNIK-OBERSTEIN, Sarit Y in SCHLINGEMANN, Reinier O., 2017, Identification of proteins associated with clinical and pathological features of proliferative diabetic retinopathy in vitreous and fibrovascular membranes. PloS one [na spletu]. 2017. Vol. 12, no. 11, e0187304, p. 1–21. [Dostopano 6 april 2025]. DOI 10.1371/journal.pone.0187304. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=19703
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Gradivo je del revije

Naslov:PloS one
Založnik:Public Library of Science
ISSN:1932-6203
COBISS.SI-ID:2005896 Novo okno

Gradivo je financirano iz projekta

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Landelijke Stichting voor Blinden en Slechtzienden (LSBS), Stichting Blinden-Penning and MaculaFonds
Številka projekta:UitZicht 2011-15
Naslov:Grant
Akronim:UitZicht
Financer:Drugi - Drug financer ali več financerjev
Program financ.:Stichting Ooglijders, Nederlandse Vereniging ter Verbetering van het Lot der Blinden and Stichting Nederlands Oogheelkundig Onderzoek
Številka projekta:2011-03
Naslov:Grant
Financer:Drugi - Drug financer ali več financerjev
Program financ.:Edward en Marianne Blaauw Fonds voor Oogheelkunde (Edward and Marianne Blaauw Fund for Ophthalmology)

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:biokemija, medicinske znanosti, proteini, diabetična retinopatija


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