Digitalni repozitorij raziskovalnih organizacij Slovenije

Izpis gradiva
A+ | A- | Pomoč | SLO | ENG

Naslov:IGF2 and IGF1R identified as novel tip cell genes in primary microvascular endothelial cell monolayers
Avtorji:ID Dallinga, Marchien G. (Avtor)
ID Yetkin-Arik, Bahar (Avtor)
ID Kayser, Richelle P. (Avtor)
ID Vogels, Ilse M.C. (Avtor)
ID Nowak-Sliwinska, Patrycja (Avtor)
ID Griffioen, Arjan W. (Avtor)
ID Noorden, Cornelis J. F. van (Avtor)
ID Klaassen, Ingeborg (Avtor)
ID Schlingemann, Reinier O. (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (8,85 MB)
MD5: 7B69B877B8FA8DA8F969F76CCA0A33F2
 
URL URL - Izvorni URL, za dostop obiščite https://doi.org/10.1007/s10456-018-9627-4
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Tip cells, the leading cells of angiogenic sprouts, were identified in cultures of human umbilical vein endothelial cells (HUVECs) by using CD34 as a marker. Here, we show that tip cells are also present in primary human microvascular endothelial cells (hMVECs), a more relevant endothelial cell type for angiogenesis. By means of flow cytometry, immunocytochemistry, and qPCR, it is shown that endothelial cell cultures contain a dynamic population of CD34+ cells with many hallmarks of tip cells, including filopodia-like extensions, elevated mRNA levels of known tip cell genes, and responsiveness to stimulation with VEGF and inhibition by DLL4. Furthermore, we demonstrate that our in vitro tip cell model can be exploited to investigate cellular and molecular mechanisms in tip cells and to discover novel targets for anti-angiogenesis therapy in patients. Small interfering RNA (siRNA) was used to knockdown gene expression of the known tip cell genes angiopoietin 2 (ANGPT2) and tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), which resulted in similar effects on tip cells and sprouting as compared to inhibition of tip cells in vivo. Finally, we identified two novel tip cell-specific genes in CD34+ tip cells in vitro: insulin-like growth factor 2 (IGF2) and IGF-1-receptor (IGF1R). Knockdown of these genes resulted in a significant decrease in the fraction of tip cells and in the extent of sprouting in vitro and in vivo. In conclusion, this study shows that by using our in vitro tip cell model, two novel essential tip cells genes are identified.
Ključne besede:Angiogenesis, tip cells, CD34, IGF2, endothelial cells, cultured cells, endothelial growth factors
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:27.06.2018
Leto izida:2018
Št. strani:str. 823-836
Številčenje:Vol. 21
PID:20.500.12556/DiRROS-19657 Novo okno
UDK:577
ISSN pri članku:0969-6970
DOI:10.1007/s10456-018-9627-4 Novo okno
COBISS.SI-ID:40464389 Novo okno
Datum objave v DiRROS:24.07.2024
Število ogledov:442
Število prenosov:295
Metapodatki:XML DC-XML DC-RDF
:
DALLINGA, Marchien G., YETKIN-ARIK, Bahar, KAYSER, Richelle P., VOGELS, Ilse M.C., NOWAK-SLIWINSKA, Patrycja, GRIFFIOEN, Arjan W., NOORDEN, Cornelis J. F. van, KLAASSEN, Ingeborg in SCHLINGEMANN, Reinier O., 2018, IGF2 and IGF1R identified as novel tip cell genes in primary microvascular endothelial cell monolayers. Angiogenesis [na spletu]. 2018. Vol. 21, p. 823–836. [Dostopano 6 april 2025]. DOI 10.1007/s10456-018-9627-4. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=19657
Kopiraj citat
  
Objavi na:Bookmark and Share


Postavite miškin kazalec na naslov za izpis povzetka. Klik na naslov izpiše podrobnosti ali sproži prenos.

Gradivo je del revije

Naslov:Angiogenesis
Skrajšan naslov:Angiogenesis
Založnik:Kluwer Academic Publishers
ISSN:0969-6970
COBISS.SI-ID:2873114 Novo okno

Gradivo je financirano iz projekta

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Landelijke Stichting voor Blinden en Slechtzienden, Algemene Nederlandse Vereniging Ter Voorkoming Van Bindheid, Stichting Blindenpenning, Novartisfonds, and the ODAS stichting
Naslov:Grant UitZicht 2013-12
Akronim:UitZicht
Financer:Drugi - Drug financer ali več financerjev
Program financ.:Rotterdamse Stichting Blindenbelangen
Številka projekta:B20130006
Naslov:Grant
Financer:Drugi - Drug financer ali več financerjev
Program financ.:Stichting Nederlands Oogheelkundig Onderzoek
Številka projekta:2013-04
Naslov:Grant
Financer:Drugi - Drug financer ali več financerjev
Program financ.:European Research Council
Številka projekta:StG-2015-680209
Financer:Drugi - Drug financer ali več financerjev
Program financ.:Dutch Cancer Society
Številka projekta:VU2014-7234

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:biokemija, angiogeneza, endotelne celice, CD34, IGF2


Nazaj