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Naslov:Cysteine cathepsins B, X and K expression in peri-arteriolar glioblastoma stem cell niches
Avtorji:ID Breznik, Barbara (Avtor)
ID Limbaeck Stanic, Clara (Avtor)
ID Kos, Janko (Avtor)
ID Khurshed, Mohammed (Avtor)
ID Hira, Vashendriya V. V. (Avtor)
ID Bošnjak, Roman (Avtor)
ID Lah Turnšek, Tamara (Avtor)
ID Noorden, Cornelis J. F. van (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (10,33 MB)
MD5: FB503791F1F20DF0F1035FC963C0B1A8
 
URL URL - Izvorni URL, za dostop obiščite https://doi.org/10.1007/s10735-018-9787-y
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Glioblastoma (GBM) is the most lethal brain tumor also due to malignant and therapy-resistant GBM stem cells (GSCs) that are localized in protecting hypoxic GSC niches. Some members of the cysteine cathepsin family of proteases have been found to be upregulated in GBM. Cathepsin K gene expression is highly elevated in GBM tissue versus normal brain and it has been suggested to regulate GSC migration out of the niches. Here, we investigated the cellular distribution of cathepsins B, X and K in GBM tissue and whether these cathepsins are co-localized in GSC niches. Therefore, we determined expression of these cathepsins in serial paraffin sections of 14 human GBM samples and serial cryostat sections of two samples using immunohistochemistry and metabolic mapping of cathepsin activity using selective fluorogenic substrates. We detected cathepsins B, X and K in peri-arteriolar GSC niches in 9 out of 16 GBM samples, which were defined by co-expression of the GSC marker CD133, the niche marker stromal-derived factor-1α (SDF-1α) and smooth muscle actin as a marker for arterioles. The expression of cathepsin B and X was detected in stromal cells and cancer cells throughout the GBM sections, whereas cathepsin K expression was more restricted to arteriole-rich regions in the GBM sections. Metabolic mapping showed that cathepsin B, but not cathepsin K is active in GSC niches. On the basis of these findings, it is concluded that cathepsins B, X and K have distinct functions in GBM and that cathepsin K is the most likely GSC niche-related cathepsin of the three cathepsins investigated.
Ključne besede:cysteine cathepsins, glioblastoma stem cells, niches, stroma, proteolytic activity
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:25.07.2018
Leto izida:2018
Št. strani:str. 481-497
Številčenje:Vol. 49, no. 5
PID:20.500.12556/DiRROS-19647 Novo okno
UDK:577.2
ISSN pri članku:1567-2379
DOI:10.1007/s10735-018-9787-y Novo okno
COBISS.SI-ID:4768847 Novo okno
Datum objave v DiRROS:24.07.2024
Število ogledov:495
Število prenosov:312
Metapodatki:XML DC-XML DC-RDF
:
BREZNIK, Barbara, LIMBAECK STANIC, Clara, KOS, Janko, KHURSHED, Mohammed, HIRA, Vashendriya V. V., BOŠNJAK, Roman, LAH TURNŠEK, Tamara in NOORDEN, Cornelis J. F. van, 2018, Cysteine cathepsins B, X and K expression in peri-arteriolar glioblastoma stem cell niches. Journal of molecular histology [na spletu]. 2018. Vol. 49, no. 5, p. 481–497. [Dostopano 6 april 2025]. DOI 10.1007/s10735-018-9787-y. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=19647
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Gradivo je del revije

Naslov:Journal of molecular histology
Založnik:Kluwer Academic Publishers
ISSN:1567-2379
COBISS.SI-ID:512519961 Novo okno

Gradivo je financirano iz projekta

Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:P1-0245-2015
Naslov:Ekotoksiologija, toksikološka genomika in karcinogeneza
Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Naslov:young researcher grant
Financer:EC - European Commission
Program financ.:Dutch Cancer Society,
Številka projekta:UVA 2014-6839

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

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