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Naslov:DNA metabarcoding uncovers fungal diversity of mixed airborne samples in Italy
Avtorji:ID Banchi, Elisa (Avtor)
ID Ametrano, Claudio Gennaro (Avtor)
ID Stanković, David (Avtor)
ID Verardo, Pierluigi (Avtor)
ID Moretti, Olga (Avtor)
ID Gabrielli, Francesca (Avtor)
ID Lazzarin, Stefania (Avtor)
ID Borney, Maria Francesca (Avtor)
ID Tassan, Francesca (Avtor)
ID Tretiach, Mauro (Avtor)
ID Pallavicini, Alberto (Avtor)
ID Muggia, Lucia (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (2,84 MB)
MD5: AB2C9C9BC032DC4F0C583AFC6258CCE6
 
URL URL - Izvorni URL, za dostop obiščite https://doi.org/10.1371/journal.pone.0194489
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Fungal spores and mycelium fragments are particles which become and remain airborne and have been subjects of aerobiological studies. The presence and the abundance of taxa in aerobiological samples can be very variable and impaired by changeable climatic conditions. Because many fungi produce mycotoxins and both their mycelium fragments and spores are potential allergens, monitoring the presence of these taxa is of key importance. So far data on exposure and sensitization to fungal allergens are mainly based on the assessment of few, easily identifiable taxa and focused only on certain environments. The microscopic method used to analyze aerobiological samples and the inconspicuous fungal characters do not allow a in depth taxonomical identification. Here, we present a first assessment of fungal diversity from airborne samples using a DNA metabarcoding analysis. The nuclear ITS2 region was selected as barcode to catch fungal diversity in mixed airborne samples gathered during two weeks in four sites of North-Eastern and Central Italy. We assessed the taxonomic composition and diversity within and among the sampled sites and compared the molecular data with those obtained by traditional microscopy. The molecular analyses provide a tenfold more comprehensive determination of the taxa than the traditional morphological inspections. Our results prove that the metabarcoding analysis is a promising approach to increases quality and sensitivity of the aerobiological monitoring. The laboratory and bioinformatic workflow implemented here is now suitable for routine, high-throughput, regional analyses of airborne fungi.
Ključne besede:fungi, fungal spores, taxonomy, sequence databases
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:20.03.2018
Leto izida:2018
Št. strani:str. 1-20
Številčenje:Vol. 13, iss. 3
PID:20.500.12556/DiRROS-19641 Novo okno
UDK:579
ISSN pri članku:1932-6203
DOI:10.1371/journal.pone.0194489 Novo okno
COBISS.SI-ID:4655951 Novo okno
Opomba:Nasl. z nasl. zaslona; Opis vira z dne 22. 3. 2018;
Datum objave v DiRROS:24.07.2024
Število ogledov:470
Število prenosov:189
Metapodatki:XML DC-XML DC-RDF
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Gradivo je del revije

Naslov:PloS one
Založnik:Public Library of Science
ISSN:1932-6203
COBISS.SI-ID:2005896 Novo okno

Gradivo je financirano iz projekta

Financer:Drugi - Drug financer ali več financerjev
Program financ.:University of Trieste
Naslov:Finanziamenti di Ateneo per progetti di Ricerca scientifica
Akronim:FRA2016

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Italian Government Commission with "Fondo Trieste"

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

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