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Naslov:Cell death is not sufficient for the restriction of potato virus Y spread in hypersensitive response-conferred resistance in potato
Avtorji:ID Lukan, Tjaša (Avtor)
ID Baebler, Špela (Avtor)
ID Pompe Novak, Maruša (Avtor)
ID Guček, Katja (Avtor)
ID Zagorščak, Maja (Avtor)
ID Coll Rius, Anna (Avtor)
ID Gruden, Kristina (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite https://www.frontiersin.org/articles/10.3389/fpls.2018.00168/abstract
 
.pdf PDF - Predstavitvena datoteka, prenos (6,13 MB)
MD5: 7D66D655D9CECEEF947FBC651F229CF0
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Hypersensitive response (HR)-conferred resistance to viral infection restricts the virus spread and is accompanied by the induction of cell death, manifested as the formation of necrotic lesions. While it is known that salicylic acid is the key component in the orchestration of the events restricting viral spread in HR, the exact function of the cell death in resistance is still unknown. We show that potato virus Y (PVY) can be detected outside the cell death zone in Ny-1-mediated HR in potato plants (cv. Rywal), observed as individual infected cells or small clusters of infected cells outside the cell death zone. By exploiting the features of temperature dependent Ny-1-mediated resistance, we confirmed that the cells at the border of the cell death zone are alive and harbor viable PVY that is able to reinitiate infection. To get additional insights into this phenomenon we further studied the dynamics of both cell death zone expansion and occurrence of viral infected cell islands outside it. We compared the response of Rywal plants to their transgenic counterparts, impaired in SA accumulation (NahG-Rywal), where the lesions occur but the spread of the virus is not restricted. We show that the virus is detected outside the cell death zone in all lesion developmental stages of HR lesions. We also measured the dynamics of lesions expansion in both genotypes. We show that while rapid lesion expansion is observed in SA-depleted plants, virus spread is even faster. On the other hand the majority of analyzed lesions slowly expand also in HR-conferred resistance opening the possibility that the infected cells are eventually engulfed by cell death zone. Taken altogether, we suggest that the HR cell death is separated from the resistance mechanisms which lead to PVY restriction in Ny-1 genetic background. We propose that HR should be regarded as a process where the dynamics of events is crucial for effectiveness of viral arrest albeit the exact mechanism conferring this resistance remains unknown.
Ključne besede:potato virus Y, salicylic acid, hypersensitive response, programmed cell death, callose deposits, necrotic lesion
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:15.02.2018
Leto izida:2018
Številčenje:str. 1-12, Vol. 9
PID:20.500.12556/DiRROS-19635 Novo okno
UDK:577
ISSN pri članku:1664-462X
DOI:10.3389/fpls.2018.00168 Novo okno
COBISS.SI-ID:4609615 Novo okno
Opomba:Nasl. z nasl. zaslona; Opis vira z dne 7. 2. 2018;
Datum objave v DiRROS:24.07.2024
Število ogledov:467
Število prenosov:379
Metapodatki:XML DC-XML DC-RDF
:
LUKAN, Tjaša, BAEBLER, Špela, POMPE NOVAK, Maruša, GUČEK, Katja, ZAGORŠČAK, Maja, COLL RIUS, Anna in GRUDEN, Kristina, 2018, Cell death is not sufficient for the restriction of potato virus Y spread in hypersensitive response-conferred resistance in potato. Frontiers in plant science [na spletu]. 2018. Vol. str. 1-12, no. 9. [Dostopano 4 april 2025]. DOI 10.3389/fpls.2018.00168. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=19635
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Gradivo je del revije

Naslov:Frontiers in plant science
Skrajšan naslov:Front. plant sci.
Založnik:Frontiers Research Foundation
ISSN:1664-462X
COBISS.SI-ID:3011663 Novo okno

Gradivo je financirano iz projekta

Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:P4-0165-2015
Naslov:Biotehnologija in sistemska biologija rastlin
Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:N4-0026-2014
Naslov:Priprava molekularnih postopkov za sistemsko analizo imunskega odgovora krompirja
Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:J4-7636-2016
Naslov:Prostorsko časovna analiza hipersenzitivnega odziva krompirja na krompirjev virus Y
Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:1000-15-0105

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

Jezik:Latinski jezik
Ključne besede:Solanum tuberosum L., Potyviridae


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