| Naslov: | Structural basis for the multitasking nature of the potato virus Y coat protein |
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| Avtorji: | ID Kežar, Andreja (Avtor)
ID Kavčič, Luka (Avtor)
ID Pólak, Martin (Avtor)
ID Nováček, Jiři (Avtor)
ID Gutiérrez-Aguirre, Ion (Avtor)
ID Tušek-Žnidarič, Magda (Avtor)
ID Coll Rius, Anna (Avtor)
ID Stare, Katja (Avtor)
ID Gruden, Kristina (Avtor)
ID Ravnikar, Maja (Avtor)
ID Pahovnik, David (Avtor)
ID Žagar, Ema (Avtor)
ID Merzel, Franci (Avtor)
ID Anderluh, Gregor (Avtor)
ID Podobnik, Marjetka (Avtor) |
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| Datoteke: |  URL - Izvorni URL, za dostop obiščite https://advances.sciencemag.org/content/5/7/eaaw3808
 PDF - Predstavitvena datoteka, prenos (4,43 MB)
MD5: 4C8117F2744A889CE9588E7002A748AF
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  KI - Kemijski inštitut
 NIB - Nacionalni inštitut za biologijo
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| Povzetek: | Potato virus Y (PVY) is among the most economically important plant pathogens. Using cryoelectron microscopy, we determined the near-atomic structure of PVY’s flexuous virions, revealing a previously unknown lumenal interplay between extended carboxyl-terminal regions of the coat protein units and viral RNA. RNA–coat protein interactions are crucial for the helical configuration and stability of the virion, as revealed by the unique near-atomic structure of RNA-free virus-like particles. The structures offer the first evidence for plasticity of the coat protein’s amino- and carboxyl-terminal regions. Together with mutational analysis and in planta experiments, we show their crucial role in PVY infectivity and explain the ability of the coat protein to perform multiple biological tasks. Moreover, the high modularity of PVY virus-like particles suggests their potential as a new molecular scaffold for nanobiotechnological applications. |
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| Ključne besede: | plant pathogens, potato virus Y, viral RNA |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 17.07.2019 |
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| Leto izida: | 2019 |
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| Št. strani: | str. 1-13 |
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| Številčenje: | Vol. 5, no. 7 |
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| PID: | 20.500.12556/DiRROS-19584  |
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| UDK: | 577.2 |
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| ISSN pri članku: | 2375-2548 |
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| DOI: | 10.1126/sciadv.aaw3808 |
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| COBISS.SI-ID: | 5135183 |
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| Opomba: | Nasl. z nasl. zaslona;
Opis vira z dne 30. 7. 2019;
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| Datum objave v DiRROS: | 23.07.2024 |
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| Število ogledov: | 303 |
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| Število prenosov: | 226 |
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| Metapodatki: |    |
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