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Naslov:IGF-binding proteins 3 and 4 are regulators of sprouting angiogenesis
Avtorji:ID Dallinga, Marchien G. (Avtor)
ID Habani, Yasmin I. (Avtor)
ID Kayser, Richelle P. (Avtor)
ID Noorden, Cornelis J. F. van (Avtor)
ID Klaassen, Ingeborg (Avtor)
ID Schlingemann, Reinier O. (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite https://link.springer.com/article/10.1007/s11033-020-05339-0
 
.pdf PDF - Predstavitvena datoteka, prenos (2,16 MB)
MD5: 1B7C5E1382650835E5762AEB89F92361
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Purpose We have previously identified insulin-like growth factor 2 (IGF2) and insulin-like growth factor 1 receptor (IGF1R) as essential proteins for tip cell maintenance and sprouting angiogenesis. In this study, we aim to identify other IGF family members involved in endothelial sprouting angiogenesis. Methods Effects on sprouting were analyzed in human umbilical vein endothelial cells (HUVECs) using the spheroid-based sprouting model, and were quantified as mean number of sprouts per spheroid and average sprout length. RNA silencing technology was used to knockdown gene expression. Recombinant forms of the ligands (IGF1 and IGF2, insulin) and the IGF-binding proteins (IGFBP) 3 and 4 were used to induce excess effects. Effects on the tip cell phenotype were analyzed by measuring the fraction of CD34+ tip cells using flow cytometry and immunohistochemistry in a 3D angiogenesis model. Experiments were performed in the presence and absence of serum. Results Knockdown of IGF2 inhibited sprouting in HUVECs, in particular when cultured in the absence of serum, suggesting that components in serum influence the signaling of IGF2 in angiogenesis in vitro. We then determined the effects of IGFBP3 and IGFBP4, which are both present in serum, on IGF2-IGF1R signaling in sprouting angiogenesis in the absence of serum: knockdown of IGFBP3 significantly reduced sprouting angiogenesis, whereas knockdown of IGFBP4 resulted in increased sprouting angiogenesis in both flow cytometry analysis and immunohistochemical analysis of the 3D angiogenesis model. Other IGF family members except INSR did not affect IGF2-IGF1R signaling. Conclusions Serum components and IGF binding proteins regulate IGF2 effects on sprouting angiogenesis. Whereas IGFBP3 acts as co-factor for IGF2-IGF1R binding, IGFBP4 inhibits IGF2 signaling.
Ključne besede:Angiogenesis, tip cells, IGF2, IGF binding proteins, endothelial cells, cultured cells, endothelial growth factors
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:04.03.2020
Leto izida:2020
Št. strani:str. 2561-2572
Številčenje:Vol. 47
PID:20.500.12556/DiRROS-19551 Novo okno
UDK:577
ISSN pri članku:0301-4851
DOI:10.1007/s11033-020-05339-0 Novo okno
COBISS.SI-ID:40738565 Novo okno
Datum objave v DiRROS:23.07.2024
Število ogledov:439
Število prenosov:304
Metapodatki:XML DC-XML DC-RDF
:
DALLINGA, Marchien G., HABANI, Yasmin I., KAYSER, Richelle P., NOORDEN, Cornelis J. F. van, KLAASSEN, Ingeborg in SCHLINGEMANN, Reinier O., 2020, IGF-binding proteins 3 and 4 are regulators of sprouting angiogenesis. Molecular biology reports [na spletu]. 2020. Vol. 47, p. 2561–2572. [Dostopano 6 april 2025]. DOI 10.1007/s11033-020-05339-0. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=19551
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Gradivo je del revije

Naslov:Molecular biology reports
Skrajšan naslov:Mol. biol. rep.
Založnik:D. Reidel Publ. Co.
ISSN:0301-4851
COBISS.SI-ID:14135769 Novo okno

Gradivo je financirano iz projekta

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Rotterdamse Stichting Blindenbelangen
Številka projekta:B20130006
Naslov:Grant
Financer:Drugi - Drug financer ali več financerjev
Program financ.:Stichting Nederlands Oogheelkundig Onderzoek
Številka projekta:2013–04
Naslov:Grant
Financer:Drugi - Drug financer ali več financerjev
Program financ.:Landelijke Stichting voor Blinden en Slechtzienden
Financer:Drugi - Drug financer ali več financerjev
Program financ.:Algemene Nederlandse Vereniging Ter Voorkoming Van Bindheid
Financer:Drugi - Drug financer ali več financerjev
Program financ.:Stichting Blindenpenning
Financer:Drugi - Drug financer ali več financerjev
Program financ.:Novartisfonds
Financer:Drugi - Drug financer ali več financerjev
Program financ.:ODAS stichting
Številka projekta:2013–12
Naslov:Grant UitZicht
Akronim:UitZicht

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:biokemija, angiogeneza, celice, beljakovine


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