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Naslov:Genome-informed design of a LAMP assay for the specific detection of the strain of ‘Candidatus Phytoplasma asteris’ phytoplasma occurring in grapevines in South Africa
Avtorji:ID Alič, Špela (Avtor)
ID Dermastia, Marina (Avtor)
ID Burger, Johan (Avtor)
ID Dickinson, Matthew (Avtor)
ID Pietersen, Gerhard (Avtor)
ID Pietersen, Gert (Avtor)
ID Dreo, Tanja (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite https://doi.org/10.1094/PDIS-10-21-2312-RE
 
.pdf PDF - Predstavitvena datoteka, prenos (435,28 KB)
MD5: 0BE5585CE8CDB158686876841F73CCA5
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Grapevine yellows is one of the most damaging phytoplasma-associated diseases worldwide. It is linked to several phytoplasma species, which can vary regionally due to phytoplasma and insect-vector diversity. Specific, rapid, and reliable detection of the grapevine yellows pathogen has an important role in phytoplasma control. The purpose of this study was to develop and validate a specific loop-mediated isothermal amplification (LAMP) assay for detection of a distinct strain of grapevine ‘Candidatus Phytoplasma asteris’ that is present in South Africa, through implementation of a genome-informed test design approach. Several freely available, user-friendly, web-based tools were coupled to design the specific LAMP assays. The criteria for selection of the assays were set for each step of the process, which resulted in four experimentally operative LAMP assays that targeted the ftsH/hflB gene region, specific to the aster yellows phytoplasma strain from South Africa. A real-time PCR was developed, targeting the same genetic region, to provide extensive validation of the LAMP assay. The validated molecular assays are highly specific to the targeted aster yellows phytoplasma strain from South Africa, with good sensitivity and reproducibility. We show a genome-informed molecular test design and an efficient validation approach for molecular tests if reference and sample materials are sparse and hard to obtain.
Ključne besede:aster yellows, fruit, LAMP, molecular detection, pathogendetection, phytoplasma, Prokaryotes
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:01.11.2022
Leto izida:2022
Št. strani:str. 2927-2839
Številčenje:Vol. 106, no. 11
PID:20.500.12556/DiRROS-19399 Novo okno
UDK:632
ISSN pri članku:0191-2917
DOI:10.1094/PDIS-10-21-2312-RE Novo okno
COBISS.SI-ID:131923203 Novo okno
Opomba:Ostali avtorji: Marina Dermastia, Johan Burger, Matthew Dickinson, Gerhard Pietersen, Gert Pietersen, and Tanja Dreo;
Datum objave v DiRROS:17.07.2024
Število ogledov:294
Število prenosov:186
Metapodatki:XML DC-XML DC-RDF
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Gradivo je del revije

Naslov:Plant disease
Skrajšan naslov:Plant dis.
Založnik:American Phytopathological Society
ISSN:0191-2917
COBISS.SI-ID:5660935 Novo okno

Gradivo je financirano iz projekta

Financer:EC - European Commission
Številka projekta:727459
Naslov:Insect-borne prokaryote-associated diseases in tropical and subtropical perennial crops
Akronim:TROPICSAFE

Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:P4-0165-2022
Naslov:Biotehnologija in sistemska biologija rastlin

Licence

Licenca:CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
Opis:Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

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