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Naslov:Clonality analysis of lymphoid proliferations using the BIOMED-2 clonality assays : a single institution experience
Avtorji:ID Koković, Ira (Avtor)
ID Jezeršek Novaković, Barbara (Avtor)
ID Škerl, Petra (Avtor)
ID Novaković, Srdjan (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (622,10 KB)
MD5: 6D86DC06D5590232C4277EA61F6C7798
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo OI - Onkološki inštitut Ljubljana
Povzetek:Background. Clonality determination in patients with lymphoproliferative disorders can improve the final diagnosis.The aim of our study was to evaluate the applicative value of standardized BIOMED-2 gene clonality assay protocolsfor the analysis of clonality of lymphocytes in a group of different lymphoid proliferations.Materials and methods. With this purpose, 121 specimens from 91 patients with suspected lymphoproliferationssubmitted for routine diagnostics from January to December 2011 were retrospectively analyzed. According to thefinal diagnosis, our series comprised 32 cases of B-cell lymphomas, 38 cases of non-Hodgkins T-cell lymphomas and51 cases of reactive lymphoid proliferations. Clonality testing was performed using the BIOMED-2 clonality assays.Results. The determined sensitivity of the TCR assay was 91.9%, while the sensitivity of the IGH assay was 74.2%. Thedetermined specificity of the IGH assay was 73.3% in the group of lymphomas and 87.2% in the group of reactivelesions. The determined specificity of the TCR assay was 62.5% in the group of lymphomas and 54.3% in the group ofreactive lesions.Conclusions. In the present study, we confirmed the utility of standardized BIOMED-2 clonality assays for the detectionof clonality in a routine diagnostical setting of non-Hodgkins lymphomas. Reactions for the detection of thecomplete IGH rearrangements and reactions for the detection of the TCR rearrangements are a good choice forclonality testing of a wide range of lymphoid proliferations and specimen types while the reactions for the detectionof incomplete IGH rearrangements have not shown any additional diagnostic value.
Ključne besede:Biomed-2, clonality analysis, lymphomas, IGH rearrangement, TCR rearrangement
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:01.06.2014
Založnik:Association of Radiology and Oncology
Leto izida:2014
Št. strani:str. 155-162
Številčenje:Vol. 48, no. 2
Izvor:Ljubljana
PID:20.500.12556/DiRROS-18660 Novo okno
UDK:602.6/.7:616.1
ISSN pri članku:1318-2099
DOI:10.2478/raon-2013-0072 Novo okno
COBISS.SI-ID:1715835 Novo okno
Avtorske pravice:by Authors
Datum objave v DiRROS:11.04.2024
Število ogledov:888
Število prenosov:206
Metapodatki:XML DC-XML DC-RDF
:
KOKOVIĆ, Ira, JEZERŠEK NOVAKOVIĆ, Barbara, ŠKERL, Petra in NOVAKOVIĆ, Srdjan, 2014, Clonality analysis of lymphoid proliferations using the BIOMED-2 clonality assays : a single institution experience. Radiology and oncology [na spletu]. 2014. Vol. 48, no. 2, p. 155–162. [Dostopano 18 april 2025]. DOI 10.2478/raon-2013-0072. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=18660
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Gradivo je del revije

Naslov:Radiology and oncology
Skrajšan naslov:Radiol. oncol.
Založnik:Slovenian Medical Society - Section of Radiology, Croatian Medical Association - Croatian Society of Radiology
ISSN:1318-2099
COBISS.SI-ID:32649472 Novo okno

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:klonalna anliza, limfomi, standardizirane metode, IGH preureditev, TCR preureditev


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