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Naslov:Expansive growth of two glioblastoma stem-like cell lines is mediated by bFGF and not by EGF
Avtorji:ID Podergajs, Neža (Avtor)
ID Brekka, Narve (Avtor)
ID Radlwimmer, Bernhard (Avtor)
ID Herold-Mende, Christel (Avtor)
ID Talasila, Krishna M. (Avtor)
ID Tiemann, Katja (Avtor)
ID Rajčević, Uroš (Avtor)
ID Lah Turnšek, Tamara (Avtor)
ID Bjerkvig, Rolf (Avtor)
ID Miletic, Hrvoje (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite http://www.degruyter.com/view/j/raon.2013.47.issue-4/raon-2013-0063/raon-2013-0063.xml
 
.pdf PDF - Predstavitvena datoteka, prenos (575,69 KB)
MD5: 7A4126C052F0159D875CA3F3AE0AB26A
 
Jezik:Angleški jezik
Tipologija:1.08 - Objavljeni znanstveni prispevek na konferenci
Organizacija:Logo OI - Onkološki inštitut Ljubljana
Povzetek:Background. Patient-derived glioblastoma (GBM) stem-like cells (GSCs) represent a valuable model for basic and therapeutic research. GSCs are usually propagated in serum-free Neural Basal medium supplemented with bFGF and EGF. Yet, the exact influence of these growth factors on GSCs is still unclear. Recently it was suggested that GBM stemlike cells with amplified EGFR should be cultured in stem cell medium without EGF, as the presence of EGF induced rapid loss of EGFR amplification. However, patient biopsies are usually taken into culture before their genomic profiles are defined. Thus, an important question remains whether GBM cells without EGFR amplification also can be cultured in stem cell medium without EGF.Meterials and methods. To address this question, we used two heterogeneous glioblastoma GSC lines (NCH421k and NCH644) that lack EGFR amplification.Results. Although both cell lines showed very low EGFR expression under standard growth conditions, bFGF stimulation induced higher expression of EGFR in NCH644. In both cell lines, expression of the stem cell markers nestin and CD133 was higher upon stimulation with bFGF compared to EGF. Importantly, bFGF stimulated the growth of both cell lines, whereas EGF had no effect. We verified that the growth stimulation by bFGF was either mediated by proliferation (NCH421k) or resistance to apoptosis (NCH644).Conclusions. We demonstrate that GSC cultures without EGFR amplification can be maintained and expanded with bFGF, while the addition of EGF has no significant effect and therefore can be omitted.
Ključne besede:glioblastoma, stem cell cultures, bFGF
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:01.12.2013
Založnik:Association of Radiology and Oncology
Leto izida:2013
Št. strani:str. 330-337, II
Številčenje:Vol. 47, no. 4
Izvor:Ljubljana
PID:20.500.12556/DiRROS-18590 Novo okno
UDK:616.1
ISSN pri članku:1318-2099
DOI:10.2478/raon-2013-0063 Novo okno
COBISS.SI-ID:30906329 Novo okno
Avtorske pravice:by Authors
Opomba:Izvleček v slov. na str. II;
Datum objave v DiRROS:03.04.2024
Število ogledov:661
Število prenosov:296
Metapodatki:XML DC-XML DC-RDF
:
PODERGAJS, Neža, BREKKA, Narve, RADLWIMMER, Bernhard, HEROLD-MENDE, Christel, TALASILA, Krishna M., TIEMANN, Katja, RAJČEVIĆ, Uroš, LAH TURNŠEK, Tamara, BJERKVIG, Rolf in MILETIC, Hrvoje, 2013, Expansive growth of two glioblastoma stem-like cell lines is mediated by bFGF and not by EGF. V : Articles from 7th Conference of experimental and translational oncology, [April, 20-24, 2013, Portorož] [na spletu]. Objavljeni znanstveni prispevek na konferenci. Ljubljana : Association of Radiology and Oncology. 2013. p. 330–337, ii. [Dostopano 18 april 2025]. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=18590
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Gradivo je del zbornika

Naslov:Articles from 7th Conference of experimental and translational oncology, [April, 20-24, 2013, Portorož]
COBISS.SI-ID:30907609 Novo okno

Gradivo je del revije

Naslov:Radiology and oncology
Skrajšan naslov:Radiol. oncol.
Založnik:Slovenian Medical Society - Section of Radiology, Croatian Medical Association - Croatian Society of Radiology
ISSN:1318-2099
COBISS.SI-ID:32649472 Novo okno

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:glioblastoma, kulture matičnih celic, bFGF


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