Digitalni repozitorij raziskovalnih organizacij Slovenije

Izpis gradiva
A+ | A- | Pomoč | SLO | ENG

Naslov:Recombinant human erythropoietin alters gene expression and stimulates proliferation of MCF-7 breast cancer cells
Avtorji:ID Trošt, Nina (Avtor)
ID Stepišnik, Tina (Avtor)
ID Berne, Sabina (Avtor)
ID Pucer Janež, Anja (Avtor)
ID Petan, Toni (Avtor)
ID Komel, Radovan (Avtor)
ID Debeljak, Nataša (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite http://www.degruyter.com/view/j/raon.2013.47.issue-4/raon-2013-0056/raon-2013-0056.xml?format=INT
 
.pdf PDF - Predstavitvena datoteka, prenos (850,15 KB)
MD5: AD2DF97204C0EF39D04939A085B1A434
 
Jezik:Angleški jezik
Tipologija:1.08 - Objavljeni znanstveni prispevek na konferenci
Organizacija:Logo OI - Onkološki inštitut Ljubljana
Povzetek:Background. Functional erythropoietin (EPO) signaling is not specific only to erythroid lineages and has been confirmed in several solid tumors, including breast. Three different isoforms of erythropoietin receptor (EPOR) have been reported, the soluble (EPOR-S) and truncated (EPOR-T) forms acting antagonistically to the functional EPOR. In this study, we investigated the effect of human recombinant erythropoietin (rHuEPO) on cell proliferation, early gene response and the expression of EPOR isoforms in the MCF-7 breast cancer cell line.Materials and methods. The MCF-7 cells were cultured with or without rHuEPO for 72 h or 10 weeks and assessed for their growth characteristics, expression of early response genes and different EPOR isoforms. The expression profile of EPOR and EPOR-T was determined in a range of breast cancer cell lines and compared with their invasive properties.Results. MCF-7 cell proliferation after rHuEPO treatment was dependent on the time of treatment and the concentration used. High rHuEPO concentrations (40 U/ml) stimulated cell proliferation independently of a preceding long-term exposure of MCF-7 cells to rHuEPO, while lower concentrations increased MCF-7 proliferation only after 10 weeks of treatment. Gene expression analysis showed activation of EGR1 and FOS, confirming the functionality of EPOR. rHuEPO treatment also slightly increased the expression of the functional EPOR isoform, which, however, persisted throughout the 10 weeks of treatment. The expression levels of EPOR-T were not influenced. There were no correlations between EPOR expression and the invasiveness of MCF-7, MDA-MB-231, Hs578T, Hs578Bst, SKBR3, T-47D and MCF-10A cell lines.Conclusions. rHuEPO modulates MCF-7 cell proliferation in time- and concentration-dependent manner. We confirmed EGR1, FOS and EPOR as transcription targets of the EPO-EPOR signaling loop, but could not correlate the expression of different EPOR isoforms with the invasiveness of breast cancer cell lines.
Ključne besede:breast cancer, erythropoietin, gene expression
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:01.12.2013
Založnik:Association of Radiology and Oncology
Leto izida:2013
Št. strani:str. 382-389
Številčenje:Vol. 47, no. 4
Izvor:Ljubljana
PID:20.500.12556/DiRROS-18552 Novo okno
UDK:616-006
ISSN pri članku:1318-2099
DOI:10.2478/raon-2013-0056 Novo okno
COBISS.SI-ID:30880985 Novo okno
Avtorske pravice:by Authors
Opomba:Izvleček v slov. na str. VI;
Datum objave v DiRROS:22.03.2024
Število ogledov:952
Število prenosov:341
Metapodatki:XML DC-XML DC-RDF
:
TROŠT, Nina, STEPIŠNIK, Tina, BERNE, Sabina, PUCER JANEŽ, Anja, PETAN, Toni, KOMEL, Radovan in DEBELJAK, Nataša, 2013, Recombinant human erythropoietin alters gene expression and stimulates proliferation of MCF-7 breast cancer cells. V : Articles from 7th Conference of experimental and translational oncology, [April, 20-24, 2013, Portorož] [na spletu]. Objavljeni znanstveni prispevek na konferenci. Ljubljana : Association of Radiology and Oncology. 2013. p. 382–389. [Dostopano 23 april 2025]. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=18552
Kopiraj citat
  
Objavi na:Bookmark and Share


Postavite miškin kazalec na naslov za izpis povzetka. Klik na naslov izpiše podrobnosti ali sproži prenos.

Gradivo je del zbornika

Naslov:Articles from 7th Conference of experimental and translational oncology, [April, 20-24, 2013, Portorož]
COBISS.SI-ID:30907609 Novo okno

Gradivo je del revije

Naslov:Radiology and oncology
Skrajšan naslov:Radiol. oncol.
Založnik:Slovenian Medical Society - Section of Radiology, Croatian Medical Association - Croatian Society of Radiology
ISSN:1318-2099
COBISS.SI-ID:32649472 Novo okno

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:rak dojke, eritropoetin, izražanje genov


Nazaj