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Naslov:In vitro and in vivo correlation of skin and cellular responses to nucleic acid delivery
Avtorji:ID Omerzel, Maša (Avtor)
ID Žnidar, Katarina (Avtor)
ID Sales Conniff, A. (Avtor)
ID Jesenko, Tanja (Avtor)
ID Markelc, Boštjan (Avtor)
ID Tur, Jared (Avtor)
ID Semenova, Nina (Avtor)
ID Kohena, Kristopher (Avtor)
ID Kranjc Brezar, Simona (Avtor)
ID Heller, Loree C. (Avtor)
ID Čemažar, Maja (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite https://www.sciencedirect.com/science/article/pii/S0753332222004772
 
.pdf PDF - Predstavitvena datoteka, prenos (7,72 MB)
MD5: BB681153CA2264247A6621319E566BA2
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo OI - Onkološki inštitut Ljubljana
Povzetek:Skin, the largest organ in the body, provides a passive physical barrier against infection and contains elements of the innate and adaptive immune systems. Skin consists of various cells, including keratinocytes, fibroblasts, endothelial cells and immune cells. This diversity of cell types could be important to gene therapies because DNA transfection could elicit different responses in different cell types. Previously, we observed the upregulation and activation of cytosolic DNA sensing pathways in several non-tumor and tumor cell types as well in tumors after the electroporation (electrotransfer) of plasmid DNA (pDNA). Based on this research and the innate immuno- genicity of skin, we correlated the effects of pDNA electrotransfer to fibroblasts and keratinocytes to mouse skin using reverse transcription real-time PCR (RT-qPCR) and several types of protein quantification. After pDNA electrotransfer, the mRNAs of the putative DNA sensors DEAD (AspGlu-Ala-Asp) box polypeptide 60 (Ddx60), absent in melanoma 2 (Aim2), Z-DNA binding protein 1 (Zbp1), interferon activated gene 202 (Ifi202), and interferon-inducible protein 204 (Ifi204) were upregulated in keratinocytes, while Ddx60, Zbp1 and Ifi204 were upregulated in fibroblasts. Increased levels of the mRNAs and proteins of several cytokines and chemokines were detected and varied based on cell type. Mouse skin experiments in vivo confirmed our in vitro results with increased expression of putative DNA sensor mRNAs and of the mRNAs and proteins of several cytokines and chemokines.
Ključne besede:DNA sensors, cytokines, electrotransfer, skin
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:01.06.2022
Založnik:Elsevier Masson SAS
Leto izida:2022
Št. strani:str. 113088-1-113088-13
Številčenje:Vol. 150
PID:20.500.12556/DiRROS-15448 Novo okno
UDK:602.6/.7
ISSN pri članku:0753-3322
DOI:10.1016/j.biopha.2022.113088 Novo okno
COBISS.SI-ID:107308035 Novo okno
Avtorske pravice:by Authors
Datum objave v DiRROS:06.09.2022
Število ogledov:1264
Število prenosov:634
Metapodatki:XML DC-XML DC-RDF
:
OMERZEL, Maša, ŽNIDAR, Katarina, SALES CONNIFF, A., JESENKO, Tanja, MARKELC, Boštjan, TUR, Jared, SEMENOVA, Nina, KOHENA, Kristopher, KRANJC BREZAR, Simona, HELLER, Loree C. in ČEMAŽAR, Maja, 2022, In vitro and in vivo correlation of skin and cellular responses to nucleic acid delivery. Biomedicine & pharmacotherapy [na spletu]. 2022. Vol. 150, p. 113088-1-113088–13. [Dostopano 18 april 2025]. DOI 10.1016/j.biopha.2022.113088. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=15448
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Gradivo je del revije

Naslov:Biomedicine & pharmacotherapy
Skrajšan naslov:Biomed. pharmacother.
Založnik:Masson
ISSN:0753-3322
COBISS.SI-ID:25098240 Novo okno

Gradivo je financirano iz projekta

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P3-0003-2022
Naslov:Razvoj in ovrednotenje novih terapij za zdravljenje malignih tumorjev

Licence

Licenca:CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
Opis:Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.
Začetek licenciranja:06.09.2022

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Jezik:Slovenski jezik
Ključne besede:senzorji DNK, citokini, elektroprenos, koža


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