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3. High-resolution Pan-European forest structure maps : an integration of earth observation and national forest inventory dataJukka Miettinen, Patricia Adame, Radim Adolt, Iciar Alberdi, Oleg Antropov, Ólafur Arnarsson, Rasmus Astrup, Ambros Berger, Jón Bogason, Gherardo Chirici, Luka Krajnc, Mitja Skudnik, 2024, zaključena znanstvena zbirka raziskovalnih podatkov Ključne besede: uneven-aged forest, lidar data, canopy height model, voxels, canopy height diversity
Objavljeno v DiRROS: 13.08.2024; Ogledov: 249; Prenosov: 559
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4. Identifying even- and uneven-aged forest stands using low-resolution nationwide lidar dataAnže Martin Pintar, Mitja Skudnik, 2024, izvirni znanstveni članek Povzetek: In uneven-aged forests, trees of different diameters, heights, and ages are located in a small area, which is due to the felling of individual trees or groups of trees, as well as small-scale natural disturbances. In this article, we present an objective method for classifying forest stands into even- and uneven-aged stands based on freely available low-resolution (with an average recording density of 5 points/m2) national lidar data. The canopy closure, dominant height, and canopy height diversity from the canopy height model and the voxels derived from lidar data were used to classify the forest stands. Both approaches for determining forest structural diversity (canopy height diversity—CHDCHM and CHDV) yielded similar results, namely two clusters of even- and uneven-aged stands, although the differences in vertical diversity between even- and uneven-aged stands were greater when using CHM. The first analysis, using CHM for the CHD assessment, estimated the uneven-aged forest area as 49.3%, whereas the second analysis using voxels estimated it as 34.3%. We concluded that in areas with low laser scanner density, CHM analysis is a more appropriate method for assessing forest stand height heterogeneity. The advantage of detecting uneven-aged structures with voxels is that we were able to detect shade-tolerant species of varying age classes beneath a dense canopy of mature, dominant trees. The CHDCHM values were estimated to be 1.83 and 1.86 for uneven-aged forests, whereas they were 1.57 and 1.58 for mature even-aged forests. The CHDV values were estimated as 1.50 and 1.62 for uneven-aged forests, while they were 1.33 and 1.48 for mature even-aged forests. The classification of stands based on lidar data was validated with data from measurements on permanent sample plots. Statistically significantly lower average values of the homogeneity index and higher values of the Shannon–Wiener index from field measurements confirm the success of the classification of stands based on lidar data as uneven-aged forests.
Ključne besede: uneven-aged forest, lidar data, canopy height model, voxels, canopy height diversity
Objavljeno v DiRROS: 13.08.2024; Ogledov: 183; Prenosov: 601
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5. Gene expression profiling of recombinant protein producing E. coli at suboptimal growth temperatureMitja Mahnič, Špela Baebler, Andrej Blejec, Špela Jalen, Kristina Gruden, Viktor Menart, Simona Jevševar, 2012, izvirni znanstveni članek Povzetek: Recent studies have revealed that at lower cultivation temperatures (25 °C) much higher percentage of correctly folded recombinant hG-CSF protein can be extracted from inclusion bodies. Hence, the goal of our research was to investigate mechanisms determining characteristics of non-classical inclusion bodies production using gene expression profiling, focusing on proteases and chaperones gene expression. Statistical analysis of microarray data showed prominent changes in energy metabolism, in metabolism of amino acids and nucleotides, as well as in biosynthesis of cofactors and secondary metabolites if the culture was grown below its optimal temperature. Moreover, 24 differentially expressed up to now known genes classified among proteases, chaperones and other heat or stress related genes. Among chaperones UspE and among proteases YaeL and YeaZ might play an important role in accumulation of correctly folded recombinant proteins. Membrane localized protease yaeL gene was found to have higher activity at 25 °C and is thus potentially functionally related to the more efficient recombinant protein production at lower temperatures. The results of this study represent advance in the understanding of recombinant protein production in E. coli. Genes potentially influencing production of recombinant protein at lower growth temperature represent basis for further research towards improvement of E. coli production strains as well as fermentation process.
Ključne besede: recombinant protein production, non-classical inclusion bodies, expression microarrays, YaeL protease, GroEL chaperone
Objavljeno v DiRROS: 05.08.2024; Ogledov: 334; Prenosov: 284
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6. Roost-site characteristics of the Mediterranean shag Phalacrocorax aristotelis desmarestii along the Slovenian coastDejan Bordjan, Matej Gamser, Aleksander Kozina, Jure Novak, Mitja Denac, 2013, izvirni znanstveni članek Povzetek: Several bird species utilize artificial structures for communal roosting. Between 26 May and 28 Jun 2012, the selection of buoys and times of departure by Mediterranean Shags Phalacrocorax aristotelis desmarestii were studied at three communal roosts within shellfish farms in the Bays of Sv. Jernej (Debeli rtič), Strunjan and Piran (Sečovlje). A total of 3,110 buoys were counted and categorized into two groups according to their shape (horizontal and vertical) and colours. The black horizontally floating buoys were of two types (barrelshaped and oval). The Shags chose to utilize the horizontally floating buoys
only, most often black and white in colour. Owing to their poorer stability and smaller standing surface, the vertically floating buoys are clearly unsuitable for them. The highest share of Shags with regard to the number of buoys of separate types was registered on black barrel-shaped buoys. As the percentage of occupied buoys was similar at all roost sites (36–39%), it was deduced that Shags distribute evenly among roosts, regardless of the number of individuals present in the Slovenian sea. At the larger roost at Debeli rtič, the percentage of adult individuals (73.5%) was greater than at Strunjan (42.5%). This could be due to the competition for better places, given that competitively stronger individuals select safer larger roosts. Between 5.42 and 9.00 hrs, 53.3% and
69.1% of Shags departed from the roost sites at Debeli rtič and Strunjan, respectively, with the majority of departures recorded between 7.50 and 8.30 hrs. They left their roosts mostly individually (48.7%) or in pairs (23.3%), at Debeli rtič predominantly in the SW (58.9%) and W (16.9%) directions, and at Strunjan in the NE (42.3%) and N (38.5%) directions.
Ključne besede: Mediterranean Shag, Phalacrocorax aristotelis desmarestii, roost-site, buoys, Slovenian coast
Objavljeno v DiRROS: 02.08.2024; Ogledov: 201; Prenosov: 95
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8. Use of hydrodynamic cavitation in (waste)water treatmentMatevž Dular, Tjaša Griessler Bulc, Ion Gutiérrez-Aguirre, Ester Heath, Tina Kosjek, Aleksandra Krivograd-Klemenčič, Martina Oder, Martin Petkovšek, Nejc Rački, Maja Ravnikar, Andrej Šarc, Brane Širok, Mojca Zupanc, Miha Žitnik, Boris Kompare, 2016, izvirni znanstveni članek Povzetek: The use of acoustic cavitation for water and wastewater treatment (cleaning) is a well known procedure. Yet, the use of hydrodynamic cavitation as a sole technique or in combination with other techniques such as ultrasound has only recently been suggested and employed.
In the first part of this paper a general overview of techniques that employ hydrodynamic cavitation for cleaning of water and wastewater is presented.
In the second part of the paper the focus is on our own most recent work using hydrodynamic cavitation for removal of pharmaceuticals (clofibric acid, ibuprofen, ketoprofen, naproxen, diclofenac, carbamazepine), toxic cyanobacteria (Microcystis aeruginosa), green microalgae (Chlorella vulgaris), bacteria (Legionella pneumophila) and viruses (Rotavirus) from water and wastewater.
As will be shown, hydrodynamic cavitation, like acoustic, can manifest itself in many different forms each having its own distinctive properties and mechanisms. This was until now neglected, which eventually led to poor performance of the technique. We will show that a different type of hydrodynamic cavitation (different removal mechanism) is required for successful removal of different pollutants.
The path to use hydrodynamic cavitation as a routine water cleaning method is still long, but recent results have already shown great potential for optimisation, which could lead to a low energy tool for water and wastewater cleaning.
Ključne besede: wastewater, cleaning, hydrodynamic cavitation, pharmaceuticals, cyanobacteria, microalgae, viruses, Legionella bacteria
Objavljeno v DiRROS: 25.07.2024; Ogledov: 184; Prenosov: 95
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9. Protocol of the study for predicting empathy during VR sessions using sensor data and machine learningEmilija Kizhevska, Kristina Šparemblek, Mitja Luštrek, 2024, izvirni znanstveni članek Povzetek: Virtual reality (VR) technology is often referred to as the ‘ultimate empathy machine’ due to its capability to immerse users in alternate perspectives and environments beyond their immediate physical reality. In this study, participants will be immersed in 3-dimensional 360˚ VR videos where actors express different emotions (sadness, happiness, anger, and anxiousness). The primary objective is to investigate the potential relationship between participants’ empathy levels and the changes in their physiological attributes. The empathy levels will be self-reported with questionnaires, and physiological attributes will be measured using different sensors. The main outcome of the study will be a machine learning model to predict a person’s empathy level based on their physiological responses while watching VR videos. Despite the existence of established methodologies and metrics in research and clinical domains, our aim is to contribute to addressing the gap of a universally accepted “gold standard” for assessing empathy. Additionally, we expect to deepen our understanding of the relationship between different emotions and psychological attributes, gender differences in empathy, and the impact of narrative context on empathic responses.
Objavljeno v DiRROS: 23.07.2024; Ogledov: 171; Prenosov: 92
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