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Electroporator for in vitro cell permeabilization
Marko Puc, Karel Flisar, Stanislav Reberšek, Damijan Miklavčič, 2001, izvirni znanstveni članek

Povzetek: The use of high voltage electric pulse technology, electroporation, in cell biology, biotechnology and medicine has attracted an enormous interest. Electroporation is a transient phenomenon that increases the permeability of cell plasma membrane. In the state of high permeability, the plasma membrane allows small and large molecules to be introduced into the cytoplasm, althoughthe cell plasma membrane represents a considerable barrier for them inits normal state. The effectiveness of electroporation depends on many parameters that can be divided into the parameters of the electric field and the parameters that define the state of cells and their surrounding i.e, temperature, osmotic pressure, etc. In this article, we present a prototype electroporator GT-1 for in vitro electropermeabilization that we have developed. Our electroporator offers a vast flexibility of parameters and can generate high and low voltage pulses, of which the latter ones are used for electrophoretic transfer of charged molecules through permeabilized cell plasma membrane.
Ključne besede: electroporation instrumentation methods, cell membrane permeabilization
Objavljeno v DiRROS: 25.01.2024; Ogledov: 197; Prenosov: 44
.pdf Celotno besedilo (239,19 KB)

46.
Tikhoff-Lindberg operation and major resections of the shoulder girdle standard procedure for limb salvage in sarcoma patients
Marko Špiler, Janez Novak, Mojca Senčar, 2000, objavljeni povzetek znanstvenega prispevka na konferenci

Objavljeno v DiRROS: 25.01.2024; Ogledov: 169; Prenosov: 41
.pdf Celotno besedilo (70,04 KB)

47.
Sentinel lymph node biopsy in patients with malignant melanoma
Marko Hočevar, Nikola Bešić, Marko Snoj, Tadeja Movrin, 2000, objavljeni povzetek znanstvenega prispevka na konferenci

Objavljeno v DiRROS: 25.01.2024; Ogledov: 157; Prenosov: 38
.pdf Celotno besedilo (78,90 KB)

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Sentinel node biopsy in breast cancer
Marko Snoj, Janez Žgajnar, Tadeja Movrin, Rastko Golouh, 2000, objavljeni povzetek znanstvenega prispevka na konferenci

Objavljeno v DiRROS: 25.01.2024; Ogledov: 167; Prenosov: 37
.pdf Celotno besedilo (78,79 KB)

50.
A paraungual tumor? - No, just tungiasis
Rastko Golouh, Marko Špiler, 2000, strokovni članek

Povzetek: Background. Tunga penetrans is a burrowing flea that is prevalent in Central and South America, the Caribbean, tropical Africa, India and Pakistan. Tungiasis results from cutaneous infestation by gravid female flea, Tunga penetrans. Case report. We report a case of tungiasis in a male who had been on a tourist expedition to Bolivia and Peru. The condition had manifested as aparaungual infiltration of a toe, that was clinically suspicious for tumor. Conclusions. Microscopic examination of the lesion and travel history led to the diagnosis of tungiasis. This is the first case report of Tunga penetrans infestation in Slovenia.
Objavljeno v DiRROS: 23.01.2024; Ogledov: 149; Prenosov: 38
.pdf Celotno besedilo (864,68 KB)

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