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Title:Identifikacija izvora gozdnega reprodukcijskega materiala bukve s pomočjo molekularnih metod
Authors:ID Westergren, Marjana (Author)
ID Bajc, Marko (Author)
ID Finžgar, Domen (Author)
ID Božič, Gregor (Author)
ID Kraigher, Hojka (Author)
Files:.pdf PDF - Presentation file, download (512,44 KB)
MD5: 44AE4EF224E9E51E55901209DBE46BD3
PID: 20.500.12556/dirros/dab9d3f3-5f72-428d-ba69-6e88edc55a5e
 
Language:Slovenian
Typology:1.01 - Original Scientific Article
Organization:Logo ZGDS - Association of Forestry Societies of Slovenia
Abstract:Od kakovosti in izvora gozdnega reprodukcijskega materiala (GRM) bodo odvisni genetska pestrost, struktura, preživetje in uspevanje sadik ter končno uspevanje in odpornost bodočega gozda. Zato mora Gozdarski inštitut Slovenije (GIS) na podlagi javnega pooblastila po Zakonu o gozdovih in zahtev zakonodaje o GRM preveriti njegov izvor pred izdajo glavnega spričevala ali na zahtevo inšpektorata kadarkoli v času trženja in uporabe. Uporaba molekularnih metod pripomore k vedenju o izvoru in genetski kakovosti GRM. GIS kontinuirano razvija znanje, infrastrukturo, gensko banko in molekularne baze podatkov za izvajanje opisanih testov. Zaradi suma o (načrtno) napačni navedbi izvora GRM, nabranega jeseni 2016 iz vsaj enega semenskega objekta, smo v predstavljeni študiji analizirali izvor in genetsko pestrost GRM iz štirih semenskih objektov bukve (Fagus sylvatica L.), v katerih je bilo seme nabrano v istem letu, ter drugih vzorcev iz Slovenske gozdne genske banke, skupaj petnajst. Za analize smo uporabili jedrne mikrosatelite, šestnajst lokusov, ki jih na GIS uporabljamo rutinsko. S tehničnega vidika so se izbrani markerji izkazali za primerne za identifikacijo posameznikov, oceno genetske pestrosti in identifikacijo domnevnega izvora. Zaradi možnosti mešanja vzorcev, pridobljenih s tal, in tehničnih potreb bomo v prihodnosti za potrebe rekonstrukcije genotipa semenskega drevesa analizirali vsaj dvanajst semen na drevo. Genetska pestrost manjših vzorcev je bila značilno manjša od tiste v velikih vzorcih. Opozarjamo, da je GRM nujno treba nabirati najmanj z v odobritvi semenskega objekta predpisanega števila dreves, da zagotovimo ustrezno veliko genetsko pestrost GRM, ki ga sadimo v gozdove. Metode razvrščanja posameznikov na podlagi Bayesove verjetnosti in filogenetska drevesa so pravilno določili izvor referenčnih vzorcev, medtem ko je bila resolucija analize glavnih komponent manjša. Vse metode, uporabljene za identifikacijo domnevnega izvora vzorcev semena, so nedvoumno pokazale, da vzorec TURs ni bil nabran v sestoju TUR, različen je tudi od preostalega analiziranega genofonda bukve v Sloveniji.
Keywords:Fagus sylvatica, gozdni reprodukcijski material, identifikacija izvora, genetska pestrost, mikrosateliti, analiza starševstva, Slovenija
Year of publishing:2017
Year of performance:2017
Number of pages:str. 328-343
Numbering:Letn. 75, št. 7/8
PID:20.500.12556/DiRROS-8091 New window
UDC:630*16+630*23:630*17(497.4)(045)=163.6
ISSN on article:0017-2723
COBISS.SI-ID:4858278 New window
Publication date in DiRROS:03.09.2017
Views:4625
Downloads:934
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:
WESTERGREN, Marjana, BAJC, Marko, FINŽGAR, Domen, BOŽIČ, Gregor and KRAIGHER, Hojka, 2017, Identifikacija izvora gozdnega reprodukcijskega materiala bukve s pomočjo molekularnih metod. Gozdarski vestnik : slovenska strokovna revija za gozdarstvo [online]. 2017. Vol. 75, no. 7/8, p. 328–343. [Accessed 3 April 2025]. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=8091
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Record is a part of a journal

Title:Gozdarski vestnik : slovenska strokovna revija za gozdarstvo
Shortened title:Gozd. vestn.
Publisher:Zveza gozdarskih društev Slovenije
ISSN:0017-2723
COBISS.SI-ID:3736834 New window

Secondary language

Language:English
Title:Identification of forest reproductive material origin of European beech using molecular methods
Abstract:Quality and origin of forest reproductive material (FRM) define the possible genetic diversity, structure, survival and development of seedlings and resilience of the future forest to stress and disturbances. The Slovenian Forestry Institute (SFI) must, based on the public authorization, in accordance to the Forest Act, The Act on Forest Reproductive Material and other legislation requirements concerning FRM, check its origin before issuing the master certificate or, on demand of the inspectorate, at any time during its marketing and use. Application of molecular methods contributes to the determination of FRM origin and its genetic quality. SFI continually develops know-how, infrastructure, gene bank, and molecular databases for performing such tests. In this study, the origin (provenance) and genetic diversity of FRM collected from four beech (Fagus sylvatica L.) seed stands in 2016 were analysed, resulting on the suspicion of (intentional) mislabelling of the origin of the FRM, and compared to samples stored in the Slovenian Forest Gene Bank. In total 15 samples were analysed using 16 nuclear microsatellite loci. From the technical point of view, the selected markers proved to be appropriate for individual tree identification, evaluation of genetic diversity, and identification of the alleged origin. Due to the possible mixing of samples, for which seed was collected from the ground, and technical needs, we will analyze in the future at least 12 seeds per tree for the needs of the seed tree genotype reconstruction. Genetic diversity of smaller samples (seed collected from less seed trees) was significantly lower than that of large ones. We would like to emphasize that FRM should be collected at least from the number of trees prescribed in the decree on approval of the seed object in order to safeguard genetic diversity of FRM. Methods of clustering using Bayesian methods and phylogenetic trees correctly determined the origin of reference samples, while the resolution of the principle component analysis was lower. All methods used for identification of the alleged origin of seed samples, unambiguously proved that TURs sample was not collected in TUR seed stand, and it also differed from the rest of the analysed beech gene pool in Slovenia. In the presented case study, the applied methods for the molecular identification of FRM origin proved to be necessary and prevented introduction of the inappropriate FRM into Slovenian forests.
Keywords:Fagus sylvatica, forest reproductive material, identification of origin, genetic diversity, microsatellites, parentage analysis, Slovenia


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