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Title:Vpliv glivnih in rastlinskih sekundarnih metabolitov na verižno reakcijo s polimerazo (PCR)
Authors:ID Thaler, Nejc (Author)
ID Bajc, Marko (Author)
Files:.pdf PDF - Presentation file, download (685,41 KB)
MD5: 01D24CF105672050D136EEB9101D79FE
PID: 20.500.12556/dirros/5a0d6b76-ab5b-45bc-b1fd-3b03261fca35
 
Language:Slovenian
Typology:1.02 - Review Article
Organization:Logo SciVie - Slovenian Forestry Institute
Abstract:Sekundarni metaboliti so organske spojine, ki jih najdemo pri glivah in rastlinah, kjer imajo vlogo obrambnih in signalnih molekul ali zagotavljajo druge selekcijske prednosti, niso pa neposredno vpleteni v rast, razvoj in razmnoževanje organizma. Pri delu s tehnikami DNA so pogosto ravno sekundarni metaboliti tisti, ki posredno ali neposredno vplivajo na uspešnost verižne reakcije s polimerazo (PCR) ali reverezno transkriptazo, in sicer tako, da otežujejo celično lizo, povzročajo razpad nukleinskih kislin ali neposredno ovirajo delovanje encima polimeraze pri pomnoževanju tarčne DNK. Glavna ovira pri aplikaciji tehnike PCR v rutinski diagnostiki je priprava visoko kvalitetne DNA brez inhibitorjev. Še posebej to velja pri izolaciji DNA iz lesnatih rastlin (Minafra in sod., 1992) in vzorcev tal (Tsai in Olson, 1991). Večina standardnih postopkov izolacije nukleinskih kislin ne odstrani rastlinskih polisaharidov in polifenolnih komponent, ki imajo lahko neposreden vpliv na pomnoževanje s PCR (Demeke in Adams, 1992). Poskusi za premostitev tovrstnih ovir vključujejo bolj dovršene metode za izolacijo nukleinskih kislin in PCR, ki vključujejo uporabo pospeševalcev PCR za odstranitev ali zmanjšanje vpliva inhibitorjev PCR. Ta pregled je osredotočen na pristope za odstranitev ali zmanjšanje vplivov rastlinskih in glivnih sekundarnih metabolitov iz vzorcev tal, različnih rastlinskih tkiv in razkrojenega lesa zaradi pomena tovrstnih raziskav za gozdarstvo in lesarstvo.
Keywords:sekundarni metaboliti, izolacija DNA, pomnoževanje DNA, verižna reakcija, polimeraza, PCR
Publication status:Published
Publication version:Version of Record
Year of publishing:2013
Number of pages:str. 25-40
Numbering:Vol. 100
PID:20.500.12556/DiRROS-7762 New window
UDC:630*84(045)=163.6
ISSN on article:2335-3112
URN:URN:NBN:SI:DOC-0V56L74N
DOI:10.20315/ASetL.100.3 New window
COBISS.SI-ID:3636646 New window
Publication date in DiRROS:12.07.2017
Views:5450
Downloads:2443
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Record is a part of a journal

Title:Acta Silvae et Ligni
Publisher:Gozdarski inštitut Slovenije, založba Silva Slovenica, Biotehniška fakulteta, Oddelek za gozdarstvo in obnovljive gozdne vire, Biotehniška fakulteta, Oddelek za lesarstvo
ISSN:2335-3112
COBISS.SI-ID:266761216 New window

Secondary language

Language:English
Title:Effect of fungal and plant secondary metabolites on polimerase chain reaction (PCR)
Abstract:Secondary metabolites are organic compounds that can be found in both fungi and plants, where they play an important role as defensive and signal molecules, or provide other kinds of advantage in natural selection, but are not directly involved in normal growth, development and reproduction of an organism. When working with DNA techniques, it is the secondary metabolites that most often affect the efficiency of polymerase chain reaction (PCR), either by hindering cell lysis, causing decomposition of nucleic acids or by direct inhibition of DNA-polymerase or reverse transcriptase amplification. The main limiting factor in the application of the PCR technique in routine diagnosis is the preparation of good quality nucleic acids isolates, free of PCR inhibitors. This is especially true in the case of woody plants (Minafra et al., 1992) and soil samples (Tsai and Olson, 1991). Most standard nucleic acids extraction procedures do not always remove contaminating plant polysaccharides or polyphenolic compounds, which can have direct inhibitory effects on subsequent PCR amplification (Demeke and Adams, 1992). Attempts to overcome these limitations included the development of more elaborate nucleic acids extraction methods and PCR, which employ PCR enhancers to eliminate or attenuate the effects of inhibitors. This review is concentrated on removal or attenuation of effects of plant and fungal secondary metabolites from soil, different plant tissues and decayed wood samples due to the significance of this type of research in forestry and wood science.


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  1. Acta Silvae et Ligni

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