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Naslov:Roles of the crotonyl-CoA carboxylase/reductase homologues in acetate assimilation and biosynthesis of immunosuppressant FK506 in Streptomyces tsukubaensis
Avtorji:ID Kosec, Gregor (Avtor)
ID Petković, Hrvoje (Avtor)
ID Baebler, Špela (Avtor)
ID Gruden, Kristina (Avtor)
ID Blažič, Marko (Avtor)
Datoteke:URL URL - Predstavitvena datoteka, za dostop obiščite http://www.microbialcellfactories.com/content/14/1/164
 
.pdf PDF - Predstavitvena datoteka, prenos (1,85 MB)
MD5: E2DA9ADA4D3D107B4E76914CD330779F
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Background In microorganisms lacking a functional glyoxylate cycle, acetate can be assimilated by alternative pathways of carbon metabolism such as the ethylmalonyl-CoA (EMC) pathway. Among the enzymes converting CoA-esters of the EMC pathway, there is a unique carboxylase that reductively carboxylates crotonyl-CoA, crotonyl-CoA carboxylase/reductase (Ccr). In addition to the EMC pathway, gene homologues of ccr can be found in secondary metabolite gene clusters that are involved in the provision of structurally diverse extender units used in the biosynthesis of polyketide natural products. The roles of multiple ccr homologues in the same genome and their potential interactions in primary and secondary metabolic pathways are poorly understood. Results In the genome of S. tsukubaensis we have identified two ccr homologues; ccr1 is located in the putative ethylmalonyl-CoA (emc) operon and allR is located on the left fringe of the FK506 cluster. AllR provides an unusual extender unit allylmalonyl-CoA (ALL) for the biosynthesis of FK506 and potentially also ethylmalonyl-CoA for the related compound FK520. We have demonstrated that in S. tsukubaensis the ccr1 gene does not have a significant role in the biosynthesis of FK506 or FK520 when cultivated on carbohydrate-based media. However, when overexpressed under the control of a strong constitutive promoter, ccr1 can take part in the biosynthesis of ethylmalonyl-CoA and thereby FK520, but not FK506. In contrast, if ccr1 is inactivated, allR is not able to sustain a functional ethylmalonyl-CoA pathway (EMC) and cannot support growth on acetate as the sole carbon source, even when constitutively expressed in the chimeric emc operon. This is somewhat surprising considering that the same chimeric emc operon results in production of FK506 as well as FK520, consistent with the previously proposed relaxed specificity of AllR for C4 and C5 substrates. Conclusions Different regulation of the expression of both ccr genes, ccr1 and allR, and their corresponding pathways EMC and ALL, respectively, in combination with the different enzymatic properties of the Ccr1 and AllR enzymes, determine an almost exclusive role of ccr1 in the EMC pathway in S. tsukubaensis, and an exclusive role of allR in the biosynthesis of FK506/FK520, thus separating the functional roles of these two genes between the primary and secondary metabolic pathways.
Ključne besede:streptomicete, Streptomyces tsukubaensis, sekundarni metaboliti, takrolimus, FK506, biosinteze poliketidov
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:14.10.2015
Leto izida:2015
Št. strani:str. 1/12-12/12
Številčenje:Vol. 14, no. 164
PID:20.500.12556/DiRROS-5792 Novo okno
ISSN:1475-2859
UDK:604.4:615.332
COBISS.SI-ID:4572536 Novo okno
Datum objave v DiRROS:29.07.2024
Število ogledov:335
Število prenosov:228
Metapodatki:XML DC-XML DC-RDF
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Gradivo je financirano iz projekta

Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:1000-08-310196
Naslov:PhD fellowship

Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:P4-0116
Naslov:Mikrobiologija in biotehnologija živil in okolja

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Government of Slovenia, Ministry of Economy
Številka projekta:3211 10 000466
Naslov:KC Brin competence centre

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