| Naslov: | Insights into the osteosarcoma microenvironment : multiscale analysis of structural and mineral heterogeneity |
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| Avtorji: | ID Rossi, Francesca (Avtor)
ID Martyna Malgorzata, Rydzyk (Avtor)
ID Barba, Luisa (Avtor)
ID Malucelli, Emil (Avtor)
ID Palamà, Maria Elisabetta Federica (Avtor)
ID Gentili, Chiara (Avtor)
ID Mastrogiacomo, Maddalena (Avtor)
ID Cedola, Alessia (Avtor)
ID Mancini, Lucia (Avtor)
ID Salomè, Murielle (Avtor)
ID Castillo Michel, Hiram (Avtor)
ID Donati, Davide Maria (Avtor)
ID Gambarotti, Marco (Avtor)
ID Lucarelli, Enrico (Avtor)
ID Fratini, Michela (Avtor)
ID Iotti, Stefano (Avtor) |
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| Datoteke: |  URL - Izvorni URL, za dostop obiščite https://www.sciencedirect.com/science/article/pii/S1742706125003101
 PDF - Predstavitvena datoteka, prenos (7,09 MB)
MD5: BB1C80C40B389E7992EE79E875768452
 DOCX - Priloga, prenos (3,49 MB)
MD5: 87D4573694F88A9F1CFD78D5A0CAE05F
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  ZAG - Zavod za gradbeništvo Slovenije
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| Povzetek: | Osteosarcoma (OS) is a malignant and heterogeneous disease that typically originates in the long bones of children and adolescents. It is characterized by the presence of immature cells having an aggressive phenotype and rapid uncontrolled proliferation. OS progression induces significant molecular and cellular changes locally within the bone, resulting in the development of an abnormal tumor microenvironment (TME). The OS TME plays a crucial role in tumor progression and development, however, the precise effects of OS on bone structure and mineralization still remain poorly understood. In this study, we examined the OS TME by analyzing samples from osteoblastic, parosteal, and periosteal osteosarcomas. Employing advanced synchrotron-based X-ray techniques, we performed a multiscale analysis to evaluate the structural and mineral complexity of tumor-affected bone. Our results revealed marked morphological differences among the osteosarcoma subtypes, while con- firming that biomineralization remains active through the production of hydroxyapatite (HA). X-ray diffraction identified two distinct hydroxyapatite crystalline phases across all samples, suggesting a critical behavior of minerals in bone. Additionally, we observed that the bone mineral structure in periosteal and parosteal osteosarcomas exhibited crystal deformations along the c-axis, whereas the osteoblastic osteosarcoma displayed a mineral profile comparable to control bone. Micro-X-ray absorption near-edge spectroscopy revealed the occurrence of a dysregulated biomineralization in the parosteal and periosteal subtypes, marked by the presence of calcium compounds different from HA, in contrast to the mature mineral state found in the osteoblastic variant. These findings highlight the complexity of osteosarcoma repercussion on bone tissue, offering new in- sights into the interactions within the OS TME. |
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| Ključne besede: | osteosarcoma, biomineralization, XANES, synchrotron-based X-ray diffraction, X-ray computed microtomography |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 01.05.2025 |
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| Založnik: | Elsevier Ltd. |
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| Leto izida: | 2025 |
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| Št. strani: | str. 193-201 |
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| Številčenje: | Vol. 199 |
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| PID: | 20.500.12556/DiRROS-22624  |
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| UDK: | 61 |
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| ISSN pri članku: | 1878-7568 |
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| DOI: | 10.1016/j.actbio.2025.04.057 |
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| COBISS.SI-ID: | 238784259 |
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| Avtorske pravice: | © 2025 The Authors |
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| Opomba: |
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| Datum objave v DiRROS: | 10.06.2025 |
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| Število ogledov: | 560 |
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| Število prenosov: | 429 |
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| Metapodatki: |    |
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