| Title: | Crayfish pet trade as a pathway for the introduction of known and novel viruses |
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| Authors: | ID Bačnik, Katarina (Author)
ID Kranjc, Luka (Author)
ID Botella, Leticia (Author)
ID Maguire, Ivana (Author)
ID Pavić, Dora (Author)
ID Patoka, Jiří (Author)
ID Dragičević, Paula (Author)
ID Blaha, Martin (Author)
ID Bielen, Ana (Author)
ID Kouba, Antonín (Author)
ID Kutnjak, Denis (Author)
ID Hudina, Sandra (Author) |
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| Files: |  URL - Source URL, visit https://doi.org/10.1016/j.jip.2025.108345
 PDF - Presentation file, download (14,36 MB)
MD5: 6BB450153FCDFB5133EFEB07AE0534C1
 ZIP - Supplement, download (1,71 MB)
MD5: D0380EB1A0DAB252894AB621FFE2856C
Description: Supplementary material
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | Expanding international pet trade has emerged as one of the main introduction pathways of aquatic invasive species, with ornamental crayfish species commonly available on the EU and global markets. Besides most frequently studied crayfish pathogens, such as Aphanomyces astaci and white spot syndrome virus (WSSV), ornamental crayfish carry associated microbial communities, which may potentially lead to the emergence of known or even novel diseases following intentional or unintentional release of animals into the wild. This is especially problematic in the case of viruses, which represent an important, yet considerably understudied, group of crayfish pathogens. Here we analyzed viromes of hepatopancreas tissue of four crayfish species acquired in the international pet trade in Europe (Procambarus clarkii, Procambarus alleni, Cherax holthuisi, and Cherax quadricarinatus) using a high throughput sequencing based metagenomic approach. Seven different known viruses were identified, which were previously either directly associated with crayfish (WSSV, Cherax quadricarinatus reovirus, chequa iflavirus, athtab bunya-like virus) or with hosts from subphylum Crustacea or invertebrates associated with freshwater environment (Shahe ispoda virus 5, Dicistroviridae sp.). Additional sequences represented 8 potential novel and divergent RNA viruses, most similar to sequences belonging to members of Picornavirales, Elliovirales, Reovirales, Hepelivirales, Tolivirales and Ghabrivirales orders. We discuss our findings in relation to their phylogenetic relationships, geographical origins, and putative pathogenicity implications. The results highlight the need for further research into the risks related to disease emergence associated with the pet trade. |
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| Keywords: | virome, RNA viruses, ornamental crayfish, pet-trade, high throughput sequencing, ecology, molecular biology, pathology, virology, environmental risk assessment |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 01.07.2025 |
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| Year of publishing: | 2025 |
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| Number of pages: | str. 1-21 |
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| Numbering: | Vol. 211, art. no. ǂ108345 |
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| PID: | 20.500.12556/DiRROS-22503  |
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| UDC: | 578 |
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| ISSN on article: | 0022-2011 |
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| DOI: | 10.1016/j.jip.2025.108345 |
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| COBISS.SI-ID: | 236953091 |
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| Note: | Soavtorji: Luka Kranjc, Leticia Botella, Ivana Maguire, Dora Pavić, Jiří Patoka, Paula Dragičević, Martin Bláha, Ana Bielen, Antonín Kouba, Denis Kutnjak, Sandra Hudina;
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| Publication date in DiRROS: | 23.05.2025 |
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| Views: | 698 |
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| Downloads: | 453 |
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