| Naslov: | [Beta]-1,3-glucanase class III promotes spread of PVY[sup]NTN and improves in planta protein production |
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| Avtorji: | ID Dobnik, David (Avtor)
ID Baebler, Špela (Avtor)
ID Kogovšek, Polona (Avtor)
ID Pompe Novak, Maruša (Avtor)
ID Štebih, Dejan (Avtor)
ID Panter, Gabriela (Avtor)
ID Janež, Nikolaja (Avtor)
ID Morisset, Dany (Avtor)
ID Žel, Jana (Avtor)
ID Gruden, Kristina (Avtor) |
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| Datoteke: |  URL - Izvorni URL, za dostop obiščite https://link.springer.com/article/10.1007%2Fs11816-013-0300-5
 PDF - Predstavitvena datoteka, prenos (388,31 KB)
MD5: F793DEC2C6E787F08A7B1AF2EFCB8D2F
URL - Izvorni URL, za dostop obiščite http://dx.doi.org/10.1007/s11816-013-0300-5
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  NIB - Nacionalni inštitut za biologijo
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| Povzetek: | Glucanases are enzymes regulating the size exclusion limit and permeability of plasmodesmata and play a role in biotic stress. In plant genomes, they are encoded as relatively large gene families divided into four classes. Most studies of plant virus interactions have focused on glucanases from classes I and II. In our study, we have evaluated the role of the β-1,3-glucanase class III (Glu-III) gene in the potato–potato virus YNTN (PVYNTN) interaction and implemented the findings to plant biotechnology application. Potato cultivars Désirée and Santé, which are tolerant and extremely resistant to PVYNTN, respectively, were stably transformed with Agrobacterium tumefaciens harbouring constructs for Glu-III overexpression. Localization of Glu-III protein in patches within the cell wall was determined by tagging the Glu-III protein with green fluorescent protein. Transgenic and non-transgenic plants were challenged with PVYNTN and its multiplication and spreading was followed. Differences in viral spread were observed between transgenic lines overexpressing Glu-III and non-transgenic lines, with stronger and faster viral spread in transgenic Désirée, and some multiplication in transgenic Santé. In addition, the ability of Glu-III to improve in planta protein production after agroinfiltration was tested. The results have shown that Glu-III overexpression enables faster spreading of vectors between cells and better protein production, which could be beneficial in improving in planta protein production system using viral vectors.
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| Ključne besede: | plant biotechnology, plant-virus interaction, potato virus Y, agroinfiltration, beta-1, 3-glucanase |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 27.08.2013 |
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| Leto izida: | 2013 |
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| Št. strani: | str. 547-555 |
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| Številčenje: | Vol. 7, iss. 4 |
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| PID: | 20.500.12556/DiRROS-21603  |
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| UDK: | 578 |
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| ISSN pri članku: | Y507-9268 |
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| DOI: | 10.1007/s11816-013-0300-5 |
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| COBISS.SI-ID: | 2897999 |
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| Datum objave v DiRROS: | 04.03.2025 |
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| Število ogledov: | 730 |
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| Število prenosov: | 840 |
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| Metapodatki: |    |
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