| Naslov: | Guidance document on multiplex real-time PCR methods : European Network of GMO Laboratories |
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| Avtorji: | ID Grohmann, Lutz (Avtor)
ID Barbante, Alessandra (Avtor)
ID Eriksson, Ronnie (Avtor)
ID Gatto, Francesco (Avtor)
ID Georgieva, Tzveta (Avtor)
ID Huber, Ingrid (Avtor)
ID Hulin, Julie (Avtor)
ID Köppel, René (Avtor)
ID Marchesi, Ugo (Avtor)
ID Marmin, Lucas (Avtor)
ID Mazzara, Marco (Avtor)
ID Narendja, Frank (Avtor)
ID Owen, Heather (Avtor)
ID Perri, Elena (Avtor)
ID Scholtens, Ingrid (Avtor)
ID Sovová, Tereza (Avtor)
ID Sowa, Slawomir (Avtor)
ID Štebih, Dejan (Avtor)
ID Weidner, Christopher (Avtor)
ID Zdeňková, Kamila (Avtor) |
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| Datoteke: |  URL - Izvorni URL, za dostop obiščite https://data.europa.eu/doi/10.2760/243914
URL - Izvorni URL, za dostop obiščite https://op.europa.eu/publication/manifestation_identifier/PUB_KJNA30708ENN
 PDF - Predstavitvena datoteka, prenos (1,65 MB)
MD5: CD406107C42F36D239E62D0C1552182D
To gradivo ima še več datotek. Celoten seznam je na voljo
spodaj. |
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| Jezik: | Angleški jezik |
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| Tipologija: | 2.12 - Končno poročilo o rezultatih raziskav |
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| Organizacija: |  NIB - Nacionalni inštitut za biologijo
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| Povzetek: | This document provides guidance for the development of new qualitative and quantitative multiplex real-time methods (mpPCR) and their implementation in a routine testing laboratory for food, feed and seeds. When implementing a mpPCR method, the laboratory should perform either an (in-house) validation or a verification of the method before its use.
The various method performance parameters of the specific requirements for mpPCR are described and examples are given. Several different aspects have been considered, including the selection of the fluorophores, the master mix and the real-time PCR instrument used. The technical aspects are examined and recommendations are made on the choice of the detection and quantification strategy. Finally, a separate chapter is dedicated to troubleshooting (e.g. underperforming method, no or poor signal strength, cross-talk).
In addition, a collection of existing mpPCR methods with emphasis on GMO detection is presented.
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 01.01.2023 |
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| Kraj izida: | Luxembourg |
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| Kraj izvedbe: | Luxembourg |
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| Založnik: | Publications Office of the European Union |
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| Leto izida: | 2021 |
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| Leto izvedbe: | 2021 |
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| Št. strani: | 1 spletni vir (1 datoteka PDF ([62] str.)) |
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| PID: | 20.500.12556/DiRROS-20262  |
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| UDK: | 577 |
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| ISBN: | 978-92-76-37820-4 |
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| DOI: | 10.2760/243914 |
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| COBISS.SI-ID: | 94024963 |
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| Opomba: | Soavtorji: Barbante A, Eriksson R, Gatto F, Georgieva T, Huber I, Hulin J, Köppel R, Marchesi U, Marmin L, Mazzara M, Narendja F, Owen H, Perri E, Scholtens I, Sovová T, Sowa S, Stebih D, Weidner C, Zdeňková K.;
Nasl. z nasl. zaslona;
Opis vira z dne 19. 1. 2022;
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| Datum objave v DiRROS: | 02.09.2024 |
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| Število ogledov: | 291 |
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| Število prenosov: | 1222 |
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| Metapodatki: |    |
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