| Title: | Guidance document on multiplex real-time PCR methods : European Network of GMO Laboratories |
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| Authors: | ID Grohmann, Lutz (Author)
ID Barbante, Alessandra (Author)
ID Eriksson, Ronnie (Author)
ID Gatto, Francesco (Author)
ID Georgieva, Tzveta (Author)
ID Huber, Ingrid (Author)
ID Hulin, Julie (Author)
ID Köppel, René (Author)
ID Marchesi, Ugo (Author)
ID Marmin, Lucas (Author)
ID Mazzara, Marco (Author)
ID Narendja, Frank (Author)
ID Owen, Heather (Author)
ID Perri, Elena (Author)
ID Scholtens, Ingrid (Author)
ID Sovová, Tereza (Author)
ID Sowa, Slawomir (Author)
ID Štebih, Dejan (Author)
ID Weidner, Christopher (Author)
ID Zdeňková, Kamila (Author) |
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| Files: |  URL - Source URL, visit https://op.europa.eu/publication/manifestation_identifier/PUB_KJNA30708ENN
URL - Source URL, visit https://data.europa.eu/doi/10.2760/243914
 PDF - Presentation file, download (1,65 MB)
MD5: CD406107C42F36D239E62D0C1552182D
This document has even more files. Complete list of files is available
below. |
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| Language: | English |
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| Typology: | 2.12 - Final Research Report |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | This document provides guidance for the development of new qualitative and quantitative multiplex real-time methods (mpPCR) and their implementation in a routine testing laboratory for food, feed and seeds. When implementing a mpPCR method, the laboratory should perform either an (in-house) validation or a verification of the method before its use.
The various method performance parameters of the specific requirements for mpPCR are described and examples are given. Several different aspects have been considered, including the selection of the fluorophores, the master mix and the real-time PCR instrument used. The technical aspects are examined and recommendations are made on the choice of the detection and quantification strategy. Finally, a separate chapter is dedicated to troubleshooting (e.g. underperforming method, no or poor signal strength, cross-talk).
In addition, a collection of existing mpPCR methods with emphasis on GMO detection is presented.
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 01.01.2023 |
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| Place of publishing: | Luxembourg |
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| Place of performance: | Luxembourg |
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| Publisher: | Publications Office of the European Union |
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| Year of publishing: | 2021 |
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| Year of performance: | 2021 |
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| Number of pages: | 1 spletni vir (1 datoteka PDF ([62] str.)) |
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| PID: | 20.500.12556/DiRROS-20262  |
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| ISBN: | 978-92-76-37820-4 |
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| UDC: | 577 |
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| DOI: | 10.2760/243914 |
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| COBISS.SI-ID: | 94024963 |
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| Note: | Soavtorji: Barbante A, Eriksson R, Gatto F, Georgieva T, Huber I, Hulin J, Köppel R, Marchesi U, Marmin L, Mazzara M, Narendja F, Owen H, Perri E, Scholtens I, Sovová T, Sowa S, Stebih D, Weidner C, Zdeňková K.;
Nasl. z nasl. zaslona;
Opis vira z dne 19. 1. 2022;
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| Publication date in DiRROS: | 02.09.2024 |
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| Views: | 9 |
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| Downloads: | 5 |
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