| Title: | Guidance on the selection and use of DNA extraction methods : JRC technical report |
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| Authors: | ID Prins, Theo W. (Author)
ID Broothaerts, Wim (Author)
ID Burns, Malcolm (Author)
ID Demšar, Tina (Author)
ID Edelmann, Sophia (Author)
ID Papazova, Nina (Author)
ID Peterseil, Verena (Author)
ID Taverniers, Isabel (Author) |
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| Files: |  URL - Source URL, visit https://publications.jrc.ec.europa.eu/repository/handle/JRC134298
 PDF - Presentation file, download (2,97 MB)
MD5: 75D1FC53D84E7DB53880994136ECF3B8
URL - Source URL, visit http://dx.doi.org/10.2760/76162
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| Language: | English |
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| Typology: | 2.12 - Final Research Report |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | DNA extraction is at the forefront of further analytical measurements on DNA targets and affects the downstream results. This report from the European Network of GMO Laboratories (ENGL) provides guidance on the selection and use of fit-for-purpose DNA extraction methods. It focusses on DNA extraction in the context of official controls on the presence and content of genetically modified organisms in food and feed. It provides guidance on protocols and selection support systems, validation approaches, assessment of DNA quality parameters and examples of practical solutions derived from collective experiences. There are many variations on the theme of DNA extraction, but there is no single protocol that works adequately across all food and feed matrices. Before using a new method in the laboratory, or in case of modifications to a protocol, validation or verification is needed to show that a chosen method is fit for purpose for use in routine analysis. This guidance is aimed to help the DNA analysis laboratories in fulfilling the standardisation requirements and support their daily operations. |
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| Keywords: | DNA extraction, GMO, detection in food and feed |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 01.01.2024 |
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| Place of publishing: | Luxembourg |
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| Place of performance: | Luxembourg |
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| Publisher: | Publications Office of the European Union |
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| Year of publishing: | 2024 |
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| Year of performance: | 2024 |
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| Number of pages: | 1 spletni vir (1 datoteka PDF (II, 67 str.)) |
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| PID: | 20.500.12556/DiRROS-20257  |
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| ISBN: | 978-92-68-11522-0 |
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| UDC: | 577.2 |
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| DOI: | 10.2760/76162 |
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| COBISS.SI-ID: | 185382659 |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 13. 2. 2024;
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| Publication date in DiRROS: | 02.09.2024 |
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| Views: | 11 |
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| Downloads: | 8 |
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