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Naslov:CD133/prominin1 is prognostic for GBM patient's survival, but inversely correlated with cysteine cathepsine' expression in gliobastoma derived spheroids
Avtorji:ID Ardebili, Seyed Yousef (Avtor)
ID Zajc, Irena (Avtor)
ID Gole, Boris (Avtor)
ID Campos, Benito (Avtor)
ID Herold-Mende, Christel (Avtor)
ID Drmota Prebil, Sara (Avtor)
ID Lah Turnšek, Tamara (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (1,01 MB)
MD5: 4792D2F6AEF0BDF5FA6608F206E19AB0
 
URL URL - Izvorni URL, za dostop obiščite https://doi.org/10.2478/v10019-011-0015-6
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Introduction. CD133 is a marker for a population of glioblastoma (GBM) and normal neural stem cells (NNSC). We aimed to reveal whether the migratory potential and differentiation of these stem cells is associated with CD133 expression and with cathepsin proteases (Cats). Materials and methods. The invasiveness of normal NNSC, GBM/CD133+ cell lines and GBM spheroids was evaluated in 3D collagen, as well as of U87-MG and normal astrocytes (NHA) grown in monolayers in 2D Matrigel. Expression of Cats B, L and S was measured at mRNA and activity levels and their relation to invasiveness, to CD133 mRNA in 26 gliomas, and to the survival of these patients. Results. The average yield of CD133+ cells from GBM samples was 9.6%. Survival of patients with higher CD133 mRNA expression was significantly shorter (p< 0.005). Invasion, associated with proteolytic degradation of matrix, was higher in normal stem cells and GBM spheroids and cells than in isolated GBM CD133+ cells. In glioma samples, there was no correlation between CD133 mRNA expression and Cat mRNAs, but there was an inverse correlation with Cat activities. Conclusions. The study confirms CD133 as a prognostic marker for the survival of GBM patients. We demonstrated that NNSC have higher invasion potential and invade the collagen matrix in a mode different from that of GBM, initiating stem cell spheres. This result could have implications for the design of new therapeutics, including protease inhibitors that specifically target invasive tumour stem cells. Increased activity of cathepsins in CD133- cells suggests their role in the invasive behaviour of GBM.
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:03.06.2011
Leto izida:2011
Št. strani:str. 102-115
Številčenje:Vol. 45, no. 2
PID:20.500.12556/DiRROS-20052 Novo okno
UDK:576
ISSN pri članku:1318-2099
DOI:10.2478/v10019-011-0015-6 Novo okno
COBISS.SI-ID:28580825 Novo okno
Opomba:Soavtorji: Irena Zajc, Boris Gole, Benito Campos, Christel Herold-Mende, Sara Dermota, Tamara T. Lah; BSDOCID158319;
Datum objave v DiRROS:05.08.2024
Število ogledov:447
Število prenosov:209
Metapodatki:XML DC-XML DC-RDF
:
ARDEBILI, Seyed Yousef, ZAJC, Irena, GOLE, Boris, CAMPOS, Benito, HEROLD-MENDE, Christel, DRMOTA PREBIL, Sara in LAH TURNŠEK, Tamara, 2011, CD133/prominin1 is prognostic for GBM patient’s survival, but inversely correlated with cysteine cathepsine’ expression in gliobastoma derived spheroids. Radiology and oncology [na spletu]. 2011. Vol. 45, no. 2, p. 102–115. [Dostopano 7 april 2025]. DOI 10.2478/v10019-011-0015-6. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=20052
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Gradivo je del revije

Naslov:Radiology and oncology
Skrajšan naslov:Radiol. oncol.
Založnik:Slovenian Medical Society - Section of Radiology, Croatian Medical Association - Croatian Society of Radiology
ISSN:1318-2099
COBISS.SI-ID:32649472 Novo okno

Gradivo je financirano iz projekta

Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:P1-0245-2009
Naslov:Ekotoksiologija, toksikološka genomika in karcinogeneza

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

Jezik:Ni določen
Naslov:[CD133/prominin1 je prognostičen za preživetje bolnikov z glioblastoma, vendar obratno korelira z izražanjem cisteinskih katepsinov]


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