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Title:CD133/prominin1 is prognostic for GBM patient's survival, but inversely correlated with cysteine cathepsine' expression in gliobastoma derived spheroids
Authors:ID Ardebili, Seyed Yousef (Author)
ID Zajc, Irena (Author)
ID Gole, Boris (Author)
ID Campos, Benito (Author)
ID Herold-Mende, Christel (Author)
ID Drmota Prebil, Sara (Author)
ID Lah Turnšek, Tamara (Author)
Files:.pdf PDF - Presentation file, download (1,01 MB)
MD5: 4792D2F6AEF0BDF5FA6608F206E19AB0
 
URL URL - Source URL, visit https://doi.org/10.2478/v10019-011-0015-6
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:Introduction. CD133 is a marker for a population of glioblastoma (GBM) and normal neural stem cells (NNSC). We aimed to reveal whether the migratory potential and differentiation of these stem cells is associated with CD133 expression and with cathepsin proteases (Cats). Materials and methods. The invasiveness of normal NNSC, GBM/CD133+ cell lines and GBM spheroids was evaluated in 3D collagen, as well as of U87-MG and normal astrocytes (NHA) grown in monolayers in 2D Matrigel. Expression of Cats B, L and S was measured at mRNA and activity levels and their relation to invasiveness, to CD133 mRNA in 26 gliomas, and to the survival of these patients. Results. The average yield of CD133+ cells from GBM samples was 9.6%. Survival of patients with higher CD133 mRNA expression was significantly shorter (p< 0.005). Invasion, associated with proteolytic degradation of matrix, was higher in normal stem cells and GBM spheroids and cells than in isolated GBM CD133+ cells. In glioma samples, there was no correlation between CD133 mRNA expression and Cat mRNAs, but there was an inverse correlation with Cat activities. Conclusions. The study confirms CD133 as a prognostic marker for the survival of GBM patients. We demonstrated that NNSC have higher invasion potential and invade the collagen matrix in a mode different from that of GBM, initiating stem cell spheres. This result could have implications for the design of new therapeutics, including protease inhibitors that specifically target invasive tumour stem cells. Increased activity of cathepsins in CD133- cells suggests their role in the invasive behaviour of GBM.
Publication status:Published
Publication version:Version of Record
Publication date:03.06.2011
Year of publishing:2011
Number of pages:str. 102-115
Numbering:Vol. 45, no. 2
PID:20.500.12556/DiRROS-20052 New window
UDC:576
ISSN on article:1318-2099
DOI:10.2478/v10019-011-0015-6 New window
COBISS.SI-ID:28580825 New window
Note:Soavtorji: Irena Zajc, Boris Gole, Benito Campos, Christel Herold-Mende, Sara Dermota, Tamara T. Lah; BSDOCID158319;
Publication date in DiRROS:05.08.2024
Views:444
Downloads:209
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ARDEBILI, Seyed Yousef, ZAJC, Irena, GOLE, Boris, CAMPOS, Benito, HEROLD-MENDE, Christel, DRMOTA PREBIL, Sara and LAH TURNŠEK, Tamara, 2011, CD133/prominin1 is prognostic for GBM patient’s survival, but inversely correlated with cysteine cathepsine’ expression in gliobastoma derived spheroids. Radiology and oncology [online]. 2011. Vol. 45, no. 2, p. 102–115. [Accessed 6 April 2025]. DOI 10.2478/v10019-011-0015-6. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=20052
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Record is a part of a journal

Title:Radiology and oncology
Shortened title:Radiol. oncol.
Publisher:Slovenian Medical Society - Section of Radiology, Croatian Medical Association - Croatian Society of Radiology
ISSN:1318-2099
COBISS.SI-ID:32649472 New window

Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P1-0245-2009
Name:Ekotoksiologija, toksikološka genomika in karcinogeneza

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Undetermined
Title:[CD133/prominin1 je prognostičen za preživetje bolnikov z glioblastoma, vendar obratno korelira z izražanjem cisteinskih katepsinov]


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