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Title:Effect of poly-[alpha], [gamma], L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles
Authors:ID Stevanović, Magdalena (Author)
ID Kovačević, Branimir (Author)
ID Nunić, Jana (Author)
ID Filipič, Metka (Author)
ID Uskoković, Dragan (Author)
Files:URL URL - Source URL, visit http://dx.doi.org/10.2147/IJN.S24889
 
.pdf PDF - Presentation file, download (2,23 MB)
MD5: 3E6433669FF18268A146B09B229227B1
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:Highly stable dispersions of nanosized silver particles were synthesized using a straightforward, cost-effective, and ecofriendly method. Nontoxic glucose was utilized as a reducing agent and poly- α, γ, L-glutamic acid (PGA), a naturally occurring anionic polymer, was used as a capping agent to protect the silver nanoparticles from agglomeration and render them biocompatible. Use of ammonia during synthesis was avoided. Our study clearly demonstrates how the concentration of the capping agent plays a major role in determining the dimensions, morphology, and stability, as well as toxicity of a silver colloidal solution. Hence, proper optimization is necessary to develop silver colloids of narrow size distribution. The samples were characterized by Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, field-emission scanning electron microscopy, transmission electron microscopy, and zeta potential measurement. MTT assay results indicated good biocompatibility of the PGA-capped silver nanoparticles. Formation of intracellular reactive oxygen species was measured spectrophotometrically using 2,7-dichlorofluorescein diacetate as a fluorescent probe, and it was shown that the PGA-capped silver nanoparticles did not induce intracellular formation of reactive oxygen species.
Keywords:green synthesis, morphology, cytotoxicity
Publication status:Published
Publication version:Version of Record
Publication date:15.11.2011
Year of publishing:2011
Number of pages:str. 2837-2847
Numbering:Vol. 6
PID:20.500.12556/DiRROS-20049 New window
UDC:577
ISSN on article:1178-2013
DOI:10.2147/IJN.S24889 New window
COBISS.SI-ID:2459983 New window
Publication date in DiRROS:05.08.2024
Views:494
Downloads:271
Metadata:XML DC-XML DC-RDF
:
STEVANOVIĆ, Magdalena, KOVAČEVIĆ, Branimir, NUNIĆ, Jana, FILIPIČ, Metka and USKOKOVIĆ, Dragan, 2011, Effect of poly-[alpha], [gamma], L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles. International journal of nanomedicine [online]. 2011. Vol. 6, p. 2837–2847. [Accessed 16 April 2025]. DOI 10.2147/IJN.S24889. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=20049
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Record is a part of a journal

Title:International journal of nanomedicine
Publisher:Dove Medical Press
ISSN:1178-2013
COBISS.SI-ID:3223828 New window

Document is financed by a project

Funder:MESTD - Ministry of Education, Science and Technological Development of Republic of Serbia
Project number:III45004
Name:Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them

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License:CC BY-NC 3.0, Creative Commons Attribution-NonCommercial 3.0 Unported
Link:http://creativecommons.org/licenses/by-nc/3.0/
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