| Title: | Salicylic acid is an indispensable component of the Ny-1 resistance-gene-mediated response against Potato virus Y infection in potato |
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| Authors: | ID Baebler, Špela (Author)
ID Witek, Kamil (Author)
ID Petek, Marko (Author)
ID Stare, Katja (Author)
ID Tušek-Žnidarič, Magda (Author)
ID Pompe Novak, Maruša (Author)
ID Renaut, Jenny (Author)
ID Szajko, K. (Author)
ID Strzelczyk-Żyta, D. (Author)
ID Marczewski, W. (Author)
ID Morgiewicz, Karolina (Author)
ID Gruden, Kristina (Author)
ID Hennig, Jacek (Author) |
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| Files: |  PDF - Presentation file, download (4,98 MB)
MD5: 0EC015FDFB4E88E185EAFD73F8C7FA8F
 URL - Source URL, visit http://dx.doi.org/10.1093/jxb/ert447
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | The purpose of the study was to investigate the role of salicylic acid (SA) signalling in Ny-1-mediated hypersensitive resistance (HR) of potato (Solanum tuberosum L.) to Potato virus Y (PVY). The responses of the Ny-1 allele in the Rywal potato cultivar and transgenic NahG-Rywal potato plants that do not accumulate SA were characterized at the cytological, biochemical, transcriptome, and proteome levels. Analysis of noninoculated and inoculated leaves revealed that HR lesions started to develop from 3 d post inoculation and completely restricted the virus spread. At the cytological level, features of programmed cell death in combination with reactive oxygen species burst were observed. In response to PVY infection, SA was synthesized de novo. The lack of SA accumulation in the NahG plants led to the disease phenotype due to unrestricted viral spreading. Grafting experiments show that SA has a critical role in the inhibition of PVY spreading in parenchymal tissue, but not in vascular veins. The whole transcriptome analysis confirmed the central role of SA in orchestrating Ny-1-mediated responses and showed that the absence of SA leads to significant changes at the transcriptome level, including a delay in activation of expression of genes known to participate in defence responses. Moreover, perturbations in the expression of hormonal signalling genes were detected, shown as a switch from SA to jasmonic acid/ethylene signalling. Viral multiplication in the NahG plants was accompanied by downregulation of photosynthesis genes and activation of multiple energy-producing pathways. |
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| Keywords: | plant-pathogen interactions, Potato virus Y, salicylic acid, whole transcriptome analysis |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 01.03.2014 |
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| Year of publishing: | 2014 |
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| Number of pages: | str. 1095-1109 |
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| Numbering: | Vol. 65, iss. 4 |
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| PID: | 20.500.12556/DiRROS-19997  |
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| UDC: | 578 |
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| ISSN on article: | 0022-0957 |
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| DOI: | 10.1093/jxb/ert447 |
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| COBISS.SI-ID: | 3027535 |
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| Publication date in DiRROS: | 01.08.2024 |
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| Views: | 303 |
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| Downloads: | 223 |
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