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Title:Evaluation of cyanobacteria biomass derived from upgrade of phycocyanin fluorescence estimation
Authors:ID Rozina, Tinkara (Author)
ID Sedmak, Bojan (Author)
ID Zupančič Justin, Maja (Author)
ID Meglič, Andrej (Author)
Files:.pdf PDF - Presentation file, download (571,80 KB)
MD5: 078C0376A56392ACB45139310AF22C93
 
URL URL - Source URL, visit https://doi.org/10.14720/abs.60.2.15683
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:The number of harmful cyanobacterial blooms has increased significantly at the global level in recent years. One of the characteristics of cyanobacteria that gives them advantage over other phytoplankton organisms are auxiliary photosynthetic pigments, such as phycocyanin. This fluorescent pigment emits light at a different wavelength as chlorophyll and can therefore be used for detection of cyanobacteria in situ. In this study we used submersible phycocyanin fluorescence sensors and compare their voltage output to concentration of extracted phycocyanin, cell counts and biovolume. The relation was linear in all three cases; however, the variability of regression line slopes between different cyanobacteria strains was high in the case of PC extract concentration and cell count. The highest uniformity in the linear fits was between fluorescence signal and biovolume therefore making it the best candidate for fluorescence sensor voltage output conversion. In the context of this work we also compared different methods for PC extraction. Modifying the equations by subtracting the absorption at 750 nm almost entirely reduces the false PC concentration estimation due to sample turbidity.
Keywords:fluorescence measurements, phycocyanin, cyanobacteria
Publication status:Published
Publication version:Version of Record
Publication date:01.01.2017
Year of publishing:2017
Number of pages:str. 21-28
Numbering:Vol. 60, št. 2
PID:20.500.12556/DiRROS-19993 New window
UDC:581
ISSN on article:1408-3671
COBISS.SI-ID:4556111 New window
Note:Besedilo v angl.;
Publication date in DiRROS:01.08.2024
Views:383
Downloads:159
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Record is a part of a journal

Title:Acta biologica slovenica : ABS
Shortened title:Acta biol. slov.
Publisher:Društvo biologov Slovenije
ISSN:1408-3671
COBISS.SI-ID:68526592 New window

Licences

License:CC BY-SA 4.0, Creative Commons Attribution-ShareAlike 4.0 International
Link:http://creativecommons.org/licenses/by-sa/4.0/
Description:This Creative Commons license is very similar to the regular Attribution license, but requires the release of all derivative works under this same license.

Secondary language

Language:Slovenian
Title:Vrednotenje biomase cianobakterij na osnovi nadgradnje ocene fluorescence fikocianina
Abstract:V zadnjih letih se število škodljivih cvetenj cianobakterij na globalni ravni močno povečuje. Ena od značilnosti cianobakterij, ki jim prinaša prednost pred ostalimi fitoplanktonskimi organizmi, so pomožna fotosintezna barvila, med katerimi prevladuje fikocianin. Fikocianin fluorescira pri drugi valovni dolžini kot klorofil, zato lahko s pomočjo meritve fluorescence ugotavljamo prisotnost cianobakterij v vodnem okolju in situ. S potopnim senzorjem fluorescence fikocianina smo opravili meritve dveh sojev cianobakterije Microcystis aeruginosa in nitaste cianobakterije vrste Arthrospira platensis. Odnos med koncentracijo ekstrahiranega fikocianina, številom celic in njihovim biovolumnom ter fluorescenco fikocianina je bil v vseh treh primerih linearen, vendar pa je bila variabilnost naklonov regresijske premice v primeru koncentracije fikocianinskega ekstrakta in števila celic med različnimi vrstami cianobakterij visoka. Najvišje ujemanje naklonov linearnih regresij je bilo med signa-lom senzorja in biovolumnom, zaradi česar je najboljši kandidat za pretvorbo izhodne napetosti fluorescenčnega senzorja v limnološko pomembno količino. V okviru tega dela smo primerjali tudi različne protokole za ekstrakcijo fikocianina. Obstoječe enačbe za pretvarjanje absorpcije v koncentracijo fikocianina smo dopolnili z odštevanjem absorpcije vzorca pri 750 nm in s tem zmanjšali zavajujočo oceno koncentracije.
Keywords:meritve fluorescence, fikocianin, cianobakterije


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