| Title: | The titre of the virus in the inoculum affects the titre of the viral RNA in the host plant and the occurrence of the disease symptoms |
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| Authors: | ID Pompe Novak, Maruša (Author)
ID Križnik, Maja (Author)
ID Gruden, Kristina (Author) |
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| Files: |  URL - Source URL, visit https://journals.matheo.si/index.php/ACSi/article/viewFile/4585/1912
 PDF - Presentation file, download (289,67 KB)
MD5: 796DBC60B0FA8FB8B80CF2863E9B03E6
URL - Source URL, visit https://www.dlib.si/details/URN:NBN:SI:doc-MGXLEA5W
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | Potato virus Y (PVY) is the most economically important potato virus, therefore extensive research is focusing on elucidation of its interaction with the host. To obtain repeatable results, strict standardization of research methods is crucial. Mechanical inoculation by rubbing sap from a PVY infected plant onto the leaf surface together with a fine abrasive powder is the most convenient way of experimental transmission of PVY to host plants. However, factors determining reproducibility of this process need to be determined. In the present study, it was shown that higher titre of the virus in the inoculum resulted in faster increase of PVYNTN RNA titre in the inoculated leaves, as well as in faster translocation of PVYNTN from inoculated leaves into upper non-inoculated leaves. The final titre of PVYNTN RNA in upper non-inoculated leaves was independent of the virus titre in the inoculum. In addition, the occurrence of the disease symptoms was followed and the dependence to the titre of the virus in the inoculum was observed. |
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| Keywords: | krompir, virus krompirja Y, mehanska inokulacija, titer virusa, inokulum, Potato virus Y, PVY, potato, mechanical inoculation, inoculum, virus titre, symptoms |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 15.02.2019 |
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| Year of publishing: | 2019 |
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| Number of pages: | str. 45-49 |
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| Numbering: | Vol. 66, no. 1 |
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| PID: | 20.500.12556/DiRROS-19966  |
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| UDC: | 577.083.2:582.930.11 |
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| ISSN on article: | 1580-3155 |
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| DOI: | 10.17344/acsi.2019.4585 |
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| COBISS.SI-ID: | 4899407 |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 29. 11. 2018;
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| Publication date in DiRROS: | 31.07.2024 |
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| Views: | 312 |
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| Downloads: | 196 |
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| Metadata: |    |
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