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Title:Preliminary experiments into colonization of microorganisms from activated sludge on different types of plastics
Authors:ID Matjašič, Tjaša (Author)
ID Dreo, Tanja (Author)
ID Samardžija, Zoran (Author)
ID Bajt, Oliver (Author)
ID Kanduč, Tjaša (Author)
ID Simčič, Tatjana (Author)
ID Mori, Nataša (Author)
Files:URL URL - Source URL, visit https://doi.org/10.14720/abs.63.1.15914
 
URL URL - Source URL, visit https://journals.uni-lj.si/abs/article/view/15914
 
.pdf PDF - Presentation file, download (678,88 KB)
MD5: 8A9BC20DE17DDAA8A5E975E81466A61B
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:The presence of plastics in the environment is currently one of the most pressing global environmental problems. Microorganisms start to form biofilms on plastic surfaces when they first come in contact with the biosphere; however, these interactions and processes are little understood, especially in freshwaters. This study aimed to better understand the colonization process of microorganisms from activated sludge on plastic materials exhibiting different surface characteristics. We inoculated synthetic fabric (PET), water bottles (PET), and plastic bags for packing vegetables and fruits (HDPE) with microorganisms from activated sludge. Mixtures of plastics and activated sludge, as well as the control, were incubated at 22-24°C in Bushnell Haas (BH) liquid medium and shaken at 120 rpm for two months. The mixtures were sub-sampled weekly and seeded into fresh BH medium with test plastic materials to avoid feeding microorganisms on dead biomass. The colonization was followed by measuring optical density (OD600) of liquid medium, by measurements of isotopic composition of carbon (δ13C) in untreated and treated plastic materials and, with in-specting the plastics surface with scanning electron microscopy (SEM). Overall, the study confirmed differences between colonizing microorganisms on different plastic material when comparing SEM micrographs of materials from the flasks inoculated with activated sludge. The texture of the HDPE bag changed during the experiment in both, control and inoculated flasks, but it is not clear whether the observed changes were due to abiotic or biotic factors. We concluded that microorganisms from activated sludge are capable of colonizing both PET and HDPE materials, and biofilm formation is most probably influenced by the chemical composition of plastics and their surface characteristics.
Keywords:biofilm, plastics, SEM, isotopic composition of carbon, co-cultivation, UV sterilization
Publication status:Published
Publication version:Version of Record
Publication date:01.07.2020
Year of publishing:2020
Number of pages:str. 45-61
Numbering:Vol. 63, no. 1
PID:20.500.12556/DiRROS-19961 New window
UDC:502/504
ISSN on article:1408-3671
DOI:10.14720/abs.63.1.15914 New window
COBISS.SI-ID:26885379 New window
Publication date in DiRROS:31.07.2024
Views:547
Downloads:391
Metadata:XML DC-XML DC-RDF
:
MATJAŠIČ, Tjaša, DREO, Tanja, SAMARDŽIJA, Zoran, BAJT, Oliver, KANDUČ, Tjaša, SIMČIČ, Tatjana and MORI, Nataša, 2020, Preliminary experiments into colonization of microorganisms from activated sludge on different types of plastics. Acta biologica slovenica : ABS [online]. 2020. Vol. 63, no. 1, p. 45–61. [Accessed 16 April 2025]. DOI 10.14720/abs.63.1.15914. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=19961
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Record is a part of a journal

Title:Acta biologica slovenica : ABS
Shortened title:Acta biol. slov.
Publisher:Društvo biologov Slovenije
ISSN:1408-3671
COBISS.SI-ID:68526592 New window

Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P1-0255-2017
Name:Združbe, interakcije in komunikacije v ekosistemih
Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P4-0165-2015
Name:Biotehnologija in sistemska biologija rastlin
Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P1-0143-2020
Name:Kroženje snovi v okolju, snovna bilanca in modeliranje okoljskih procesov ter ocena tveganja

Licences

License:CC BY-SA 4.0, Creative Commons Attribution-ShareAlike 4.0 International
Link:http://creativecommons.org/licenses/by-sa/4.0/
Description:This Creative Commons license is very similar to the regular Attribution license, but requires the release of all derivative works under this same license.

Secondary language

Language:Slovenian
Title:Preliminarni poskusi kolonizacije različnih tipov plastike z mikroorganizmi iz aktivnega blata
Keywords:biofilm, plastika, SEM, izotopska sestava ogljika, ko-kultivacija, UV sterilizacija


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