| Title: | Localization patterns of cathepsins K and X and their predictive value in glioblastoma |
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| Authors: | ID Breznik, Barbara (Author)
ID Limbaeck Stanic, Clara (Author)
ID Porčnik, Andrej (Author)
ID Blejec, Andrej (Author)
ID Koprivnikar Krajnc, Miha (Author)
ID Bošnjak, Roman (Author)
ID Kos, Janko (Author)
ID Noorden, Cornelis J. F. van (Author)
ID Lah Turnšek, Tamara (Author) |
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| Files: |  PDF - Presentation file, download (1,91 MB)
MD5: 8602B867411F52C64FECAB367B26CD69
 URL - Source URL, visit https://doi.org/10.2478/raon-2018-0040
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | Background
Glioblastoma is a highly aggressive central nervous system neoplasm characterized by extensive infiltration of malignant cells into brain parenchyma, thus preventing complete tumor eradication. Cysteine cathepsins B, S, L and K are involved in cancer progression and are overexpressed in glioblastoma. We report here for the first time that cathepsin X mRNA and protein are also abundantly present in malignant glioma.
Materials and methods
Gene expression of cathepsins K and X was analyzed using publically-available tran-scriptomic datasets and correlated with glioma grade and glioblastoma subtype. Kaplan-Maier survival analysis was performed to evaluate the predictive value of cathepsin K and X mRNA expression. Cathepsin protein expression was localized and semi-quantified in tumor tissues by immunohistochemistry.
Results
Highest gene expression of cathepsins K and X was found in glioblastoma, in particular in the mesenchymal subtype. Overall, high mRNA expression of cathepsin X, but not that of cathepsin K, correlated with poor patients’ survival. Cathepsin K and X proteins were abundantly and heterogeneously expressed in glioblastoma tissue. Immuno-labeling of cathepsins K and X was observed in areas of CD133-positive glioblastoma stem cells, localized around arterioles in their niches that also expressed SDF-1α and CD68. mRNA levels of both cathepsins K and X correlated with mRNA levels of markers of glioblastoma stem cells and their niches.
Conclusions
The presence of both cathepsins in glioblastoma stem cell niche regions indicates their possible role in regulation of glioblastoma stem cell homing in their niches. The clinical relevance of this data needs to be elaborated in further prospective studies. |
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| Keywords: | cathepsins, glioblastoma, immunohistochemistry, patient survival, cancer stem cell niches |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 01.12.2018 |
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| Year of publishing: | 2018 |
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| Number of pages: | str. 433-442, VI |
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| Numbering: | Vol. 52, no. 4 |
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| PID: | 20.500.12556/DiRROS-19751  |
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| UDC: | 577.2 |
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| ISSN on article: | 1318-2099 |
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| DOI: | 10.2478/raon-2018-0040 |
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| COBISS.SI-ID: | 4873295 |
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| Publication date in DiRROS: | 24.07.2024 |
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| Views: | 378 |
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| Downloads: | 248 |
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