| Title: | The use of digital PCR to improve the application of quantitative molecular diagnostic methods for tuberculosis |
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| Authors: | ID Devonshire, Alison S. (Author)
ID Pavšič, Jernej (Author)
ID Milavec, Mojca (Author)
ID Žel, Jana (Author) |
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| Files: |  PDF - Presentation file, download (737,71 KB)
MD5: B1342D9E975D097EBFE27F2F095C7D11
 URL - Source URL, visit https://doi.org/10.1186/s12879-016-1696-7
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | Background
Real-time PCR (qPCR) based methods, such as the Xpert MTB/RIF, are increasingly being used to diagnose tuberculosis (TB). While qualitative methods are adequate for diagnosis, the therapeutic monitoring of TB patients requires quantitative methods currently performed using smear microscopy. The potential use of quantitative molecular measurements for therapeutic monitoring has been investigated but findings have been variable and inconclusive. The lack of an adequate reference method and reference materials is a barrier to understanding the source of such disagreement. Digital PCR (dPCR) offers the potential for an accurate method for quantification of specific DNA sequences in reference materials which can be used to evaluate quantitative molecular methods for TB treatment monitoring.
Methods
To assess a novel approach for the development of quality assurance materials we used dPCR to quantify specific DNA sequences in a range of prototype reference materials and evaluated accuracy between different laboratories and instruments. The materials were then also used to evaluate the quantitative performance of qPCR and Xpert MTB/RIF in eight clinical testing laboratories.
Results
dPCR was found to provide results in good agreement with the other methods tested and to be highly reproducible between laboratories without calibration even when using different instruments. When the reference materials were analysed with qPCR and Xpert MTB/RIF by clinical laboratories, all laboratories were able to correctly rank the reference materials according to concentration, however there was a marked difference in the measured magnitude.
Conclusions
TB is a disease where the quantification of the pathogen could lead to better patient management and qPCR methods offer the potential to rapidly perform such analysis. However, our findings suggest that when precisely characterised materials are used to evaluate qPCR methods, the measurement result variation is too high to determine whether molecular quantification of Mycobacterium tuberculosis would provide a clinically useful readout. The methods described in this study provide a means by which the technical performance of quantitative molecular methods can be evaluated independently of clinical variability to improve accuracy of measurement results. These will assist in ultimately increasing the likelihood that such approaches could be used to improve patient management of TB. |
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| Keywords: | digital PCR, diagnostics |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 03.08.2016 |
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| Year of publishing: | 2016 |
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| Number of pages: | str. 1-10 |
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| Numbering: | Vol. 16, no. 366 |
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| PID: | 20.500.12556/DiRROS-19711  |
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| UDC: | 577.2 |
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| ISSN on article: | 1471-2334 |
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| DOI: | 10.1186/s12879-016-1696-7 |
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| COBISS.SI-ID: | 3956303 |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 5. 8. 2016;
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| Publication date in DiRROS: | 25.07.2024 |
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| Views: | 296 |
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| Downloads: | 173 |
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