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Title:Metabolic consequences of infection of grapevine (Vitis vinifera L.) cv. ʺModra frankinjaʺ with flavescence dorée phytoplasma
Authors:ID Prezelj, Nina (Author)
ID Covington, Elizabeth (Author)
ID Roitsch, Thomas (Author)
ID Gruden, Kristina (Author)
ID Fragner, Lena (Author)
ID Weckwerth, Wolfram (Author)
ID Chersicola, Marko (Author)
ID Vodopivec, Maja (Author)
ID Dermastia, Marina (Author)
Files:.pdf PDF - Presentation file, download (2,49 MB)
MD5: DB20770811D404D3CB21A17EC3A8E637
 
URL URL - Source URL, visit https://doi.org/10.3389/fpls.2016.00711
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:Flavescence dorée, caused by the quarantine phytoplasma FDp, represents the most devastating of the grapevine yellows diseases in Europe. In an integrated study we have explored the FDp–grapevine interaction in infected grapevines of cv. “Modra frankinja” under natural conditions in the vineyard. In FDp-infected leaf vein-enriched tissues, the seasonal transcriptional profiles of 14 genes selected from various metabolic pathways showed an FDp-specific plant response compared to other grapevine yellows and uncovered a new association of the SWEET17a vacuolar transporter of fructose with pathogens. Non-targeted metabolome analysis from leaf vein-enriched tissues identified 22 significantly changed compounds with increased levels during infection. Several metabolites corroborated the gene expression study. Detailed investigation of the dynamics of carbohydrate metabolism revealed significant accumulation of sucrose and starch in the mesophyll of FDp-infected leaves, as well as significant up-regulation of genes involved in their biosynthesis. In addition, infected leaves had high activities of ADP-glucose pyrophosphorylase and, more significantly, sucrose synthase. The data support the conclusion that FDp infection inhibits phloem transport, resulting in accumulation of carbohydrates and secondary metabolites that provoke a source-sink transition and defense response status.
Keywords:fructose utilization, gene expression, invertase, metabolome, soluble sugars, starch, sucrose synthase, SWEET, VvDMR6
Publication status:Published
Publication version:Version of Record
Publication date:23.05.2016
Year of publishing:2016
Number of pages:str. 1-19
Numbering:Vol. 7
PID:20.500.12556/DiRROS-19689 New window
UDC:632
ISSN on article:1664-462X
DOI:10.3389/fpls.2016.00711 New window
COBISS.SI-ID:3870031 New window
Note:Nasl. z nasl. zaslona; Opis vira z dne 13. 5. 2016;
Publication date in DiRROS:25.07.2024
Views:515
Downloads:351
Metadata:XML DC-XML DC-RDF
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PREZELJ, Nina, COVINGTON, Elizabeth, ROITSCH, Thomas, GRUDEN, Kristina, FRAGNER, Lena, WECKWERTH, Wolfram, CHERSICOLA, Marko, VODOPIVEC, Maja and DERMASTIA, Marina, 2016, Metabolic consequences of infection of grapevine (Vitis vinifera L.) cv. ʺModra frankinjaʺ with flavescence dorée phytoplasma. Frontiers in plant science [online]. 2016. Vol. 7, p. 1–19. [Accessed 4 April 2025]. DOI 10.3389/fpls.2016.00711. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=19689
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Record is a part of a journal

Title:Frontiers in plant science
Shortened title:Front. plant sci.
Publisher:Frontiers Research Foundation
ISSN:1664-462X
COBISS.SI-ID:3011663 New window

Document is financed by a project

Funder:EC - European Commission
Project number:262032
Name:COST-EFFECTIVE HAND-HELD DEVICE FOR RAPID IN-FIELD DETECTION OF FLAVENSCENCE DOREE PHYTOPLASMA IN GRAPEVINES
Acronym:VITISENS
Funder:ARIS - Slovenian Research and Innovation Agency
Project number:1000-09-310032
Funder:Other - Other funder or multiple funders
Funding programme:Ministry of Education, Youth and Sports of CR within the National Sustainability Program I (NPU I)
Project number:LO1415
Name:Grant

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

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