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Naslov:Loop-mediated isothermal amplification : rapid molecular detection of virulence genes associated with avian pathogenic Escherichia coli in poultry
Avtorji:ID Kogovšek, Polona (Avtor)
ID Ambrožič, Jerneja (Avtor)
ID Dovč, Alenka (Avtor)
ID Dreo, Tanja (Avtor)
ID Hristov, Hristo (Avtor)
ID Krapež, Uroš (Avtor)
ID Ravnikar, Maja (Avtor)
ID Slavec, Brigita (Avtor)
ID Lotrič, Marjetka (Avtor)
ID Žel, Jana (Avtor)
ID Zorman-Rojs, Olga (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite https://academic.oup.com/ps/advance-article/doi/10.3382/ps/pey516/5205916
 
.pdf PDF - Predstavitvena datoteka, prenos (177,60 KB)
MD5: 6F2A6B33A5AC5DC87C1FE69BA158F747
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Infections with pathogenic Escherichia colican lead to different animal- and human-associated diseases. E. coliinfections are common in intensive poultry farming, and important economic losses can be expected during infections with avian pathogenic E. coli(APEC) strains followed by colibacillosis. Loop-mediated isothermal amplification (LAMP) assays were developed for rapid detection of 3 APEC-associated virulence genes: sitA, traT, and ompT. All 3 LAMP assays are shown to be specific, repeatable, and reproducible. High sensitivities of the assays are shown, where as few as 1,000 bacterial cells/mL can be detected in different matrices. On-site applicability of this LAMP method is demonstrated through testing of different sample types, from animal swabs and tissues, and from environmental samples collected from 6 commercial poultry farms. All 3 virulence genes were detected at high rates (above 85%) in samples from layer and broiler chickens with clinical signs and, interestingly, high prevalence of those genes was detected also in samples collected from clinically healthy broiler flock (above 75%) while lower prevalence was observed in remaining 3 clinically healthy chicken flocks (less than 75%). Importantly, these virulence genes were detected in almost all of the air samples from 11 randomly selected poultry houses, indicating air as an important route of E. colispread. Three LAMP assays that target APEC-associated virulence genes are shown to be sensitive and robust and are therefore applicable for rapid on-site testing of various sample types, from animal swabs to air. This on-site LAMP testing protocol offers rapid diagnostics, with results obtained in <35 min, and it can be applied to other important microorganisms to allow the required prompt measures to be taken.
Ključne besede:APEC virulence genes, isothermal amplification LAMP, on-site detection, colibacillosis
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:01.03.2019
Leto izida:2018
Št. strani:str. 1500-1510
Številčenje:Vol. 98, no. 3
PID:20.500.12556/DiRROS-19652 Novo okno
UDK:577.2
ISSN pri članku:0032-5791
DOI:10.3382/ps/pey516 Novo okno
COBISS.SI-ID:4878671 Novo okno
Datum objave v DiRROS:24.07.2024
Število ogledov:345
Število prenosov:150
Metapodatki:XML DC-XML DC-RDF
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Gradivo je del revije

Naslov:Poultry science
Skrajšan naslov:Poultry sci.
Založnik:Oxford University Press
ISSN:0032-5791
COBISS.SI-ID:5679367 Novo okno

Gradivo je financirano iz projekta

Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:P4-0165-2015
Naslov:Biotehnologija in sistemska biologija rastlin

Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:V4-1401-2014
Naslov:Obvladovanje okužb z E. coli pri perutnini: Določitev kritičnih mest vnosa bakterije E.coli, vključno z E. coli z ESBL v reje perutnine in študija preventivnih ukrepov za zmanjševanje porabe protimikrobnih zdravil

Financer:EC - European Commission
Številka projekta:613908
Naslov:Development of Cost efficient Advanced DNA-based methods for specific Traceability issues and High Level On-site applicatioNs
Akronim:DECATHLON

Licence

Licenca:CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
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