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Title:Surveillance of human enteric viruses in coastal waters using concentration with methacrylate monolithic supports prior to detection by RT-qPCR
Authors:ID Gonçalves, José (Author)
ID Gutiérrez-Aguirre, Ion (Author)
ID Balasubramanian, Mukundh Narayanan (Author)
ID Zagorščak, Maja (Author)
ID Ravnikar, Maja (Author)
ID Turk, Valentina (Author)
Files:.pdf PDF - Presentation file, download (1,12 MB)
MD5: 93B0F6B497D955B83B66201F44F1347A
 
URL URL - Source URL, visit https://doi.org/10.1016/j.marpolbul.2018.01.040
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:This is the first surveillance study using methacrylate monolithic supports to concentrate environmental coastal water samples, prior to molecular target detection by RT-qPCR. Rotaviruses (RoV) and Noroviruses (NoV) were monitored in a polluted area at the Bay of Koper (Gulf of Trieste, Northern Adriatic Sea) and at a nearby bathing area and mussel farm areas. RoV and NoV are released into the Bay of Koper, with higher rates close to the discharge of the wastewater treatment plant, however, they can be detected at recreational and mussel farming areas. Our results showed that water bodies considered safe based on FC concentrations, can still have low, yet potentially infective, concentrations of human viruses.
Keywords:Rotavirus, Norovirus, faecal coliforms, RT-qPCR, methacrylate monolithic columns, enteric virus concentration
Publication status:Published
Publication version:Version of Record
Publication date:01.03.2018
Year of publishing:2018
Number of pages:str. 307-317
Numbering:Vol. 128
PID:20.500.12556/DiRROS-19637 New window
UDC:579
ISSN on article:0025-326X
DOI:10.1016/j.marpolbul.2018.01.040 New window
COBISS.SI-ID:4611663 New window
Publication date in DiRROS:24.07.2024
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Record is a part of a journal

Title:Marine pollution bulletin
Shortened title:Mar. Pollut. Bull.
Publisher:Macmillan
ISSN:0025-326X
COBISS.SI-ID:25902336 New window

Document is financed by a project

Funder:EC - European Commission
Project number:607150
Name:Ecohydrological interfaces as critical hotspots for transformations of ecosystem exchange fluxes and biogeochemical cycling
Acronym:INTERFACES

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P1-0237-2015
Name:Raziskave obalnega morja

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P4-0165-2015
Name:Biotehnologija in sistemska biologija rastlin

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License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

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