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Title:Transmission modes affect the population structure of potato virus Y in potato
Authors:ID Silva, Washington da (Author)
ID Kutnjak, Denis (Author)
ID Xu, Yi (Author)
ID Xu, Yimin (Author)
ID Giovannoni, James (Author)
ID Elena, Santiago F. (Author)
ID Gray, Stewart (Author)
Files:URL URL - Source URL, visit https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1008608
 
.pdf PDF - Presentation file, download (3,12 MB)
MD5: F5861599DCAB08E6D4C537B1CB9A7083
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:Transmission is a crucial part of a viral life cycle and transmission mode can have an important impact on virus biology. It was demonstrated that transmission mode can influence the virulence and evolution of a virus; however, few empirical data are available to describe the direct underlying changes in virus population structure dynamics within the host. Potato virus Y (PVY) is an RNA virus and one of the most damaging pathogens of potato. It comprises several genetically variable strains that are transmitted between plants via different transmission modes. To investigate how transmission modes affect the within-plant viral population structure, we have used a deep sequencing approach to examine the changes in the genetic structure of populations (in leaves and tubers) of three PVY strains after successive passages by horizontal (aphid and mechanical) and vertical (via tubers) transmission modes. Nucleotide diversities of viral populations were significantly influenced by transmission modes; lineages transmitted by aphids were the least diverse, whereas lineages transmitted by tubers were the most diverse. Differences in nucleotide diversities of viral populations between leaves and tubers were transmission mode-dependent, with higher diversities in tubers than in leaves for aphid and mechanically transmitted lineages. Furthermore, aphid and tuber transmissions were shown to impose stronger genetic bottlenecks than mechanical transmission. To better understand the structure of virus populations within the host, transmission mode, movement of the virus within the host, and the number of replication cycles after transmission event need to be considered. Collectively, our results suggest a significant impact of virus transmission modes on the within-plant diversity of virus populations and provide quantitative fundamental data for understanding how transmission can shape virus diversity in the natural ecosystems, where different transmission modes are expected to affect virus population structure and consequently its evolution.
Publication status:Published
Publication version:Version of Record
Publication date:23.06.2020
Year of publishing:2020
Number of pages:str. e1008608, 1-23
Numbering:16, 6
PID:20.500.12556/DiRROS-19600 New window
UDC:577
ISSN on article:1553-7366
DOI:10.1371/journal.ppat.1008608 New window
COBISS.SI-ID:21858051 New window
Publication date in DiRROS:22.07.2024
Views:430
Downloads:297
Metadata:XML DC-XML DC-RDF
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SILVA, Washington da, KUTNJAK, Denis, XU, Yi, XU, Yimin, GIOVANNONI, James, ELENA, Santiago F. and GRAY, Stewart, 2020, Transmission modes affect the population structure of potato virus Y in potato. PLOS pathogens [online]. 2020. Vol. 16, no. 6, p. e1008608, 1–23. [Accessed 7 April 2025]. DOI 10.1371/journal.ppat.1008608. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=19600
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Record is a part of a journal

Title:PLOS pathogens
Publisher:Public Library of Science
ISSN:1553-7366
COBISS.SI-ID:513029401 New window

Licences

License:CC0 1.0, Creative Commons CC0 1.0 Universal
Link:https://creativecommons.org/publicdomain/zero/1.0/deed.en
Description:CC Zero enables scientists, educators, artists and other creators and owners of copyright- or database-protected content to waive those interests in their works and thereby place them as completely as possible in the public domain, so that others may freely build upon, enhance and reuse the works for any purposes without restriction under copyright or database law.

Secondary language

Language:Slovenian
Keywords:virus Y krompirja, visokozmogljivo sekvenciranje, populacijska genetika, načini prenosa


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