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Title:2D and 3D in vitro assays to quantify the invasive behavior of glioblastoma stem cells in response to SDF-1[alpha]
Authors:ID Hira, Vashendriya V. V. (Author)
ID Breznik, Barbara (Author)
ID Noorden, Cornelis J. F. van (Author)
ID Lah Turnšek, Tamara (Author)
ID Molenaar, Remco J. (Author)
Files:URL URL - Source URL, visit http://dx.doi.org/10.2144/btn-2020-0046
 
.pdf PDF - Presentation file, download (1,58 MB)
MD5: 1ED87956B30889148329277B07AB1EF4
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:Invasion is a hallmark of cancer and therefore in vitro invasion assays are important tools in cancer research. We aimed to describe in vitro 2D transwell assays and 3D spheroid assays to quantitatively determine the invasive behavior of glioblastoma stem cells in response to the chemoattractant SDF-1α. Matrigel was used as a matrix in both assays. We demonstrated quantitatively that SDF-1α increased invasive behavior of glioblastoma stem cells in both assays. We conclude that the 2D transwell invasion assay is easy to perform, fast and less complex whereas the more time-consuming 3D spheroid invasion assay is physiologically closer to the in vivo situation.
Keywords:2D transwell invasion assay, 3D spheroid invasion assay, cancer cell, cellular invasion, glioblastoma stem cells
Publication status:Published
Publication version:Version of Record
Publication date:01.09.2020
Year of publishing:2020
Number of pages:str. 339-346
Numbering:Vol. 69, no. 5
PID:20.500.12556/DiRROS-19525 New window
UDC:577.2
ISSN on article:0736-6205
DOI:10.2144/btn-2020-0046 New window
COBISS.SI-ID:26930691 New window
Publication date in DiRROS:22.07.2024
Views:517
Downloads:291
Metadata:XML DC-XML DC-RDF
:
HIRA, Vashendriya V. V., BREZNIK, Barbara, NOORDEN, Cornelis J. F. van, LAH TURNŠEK, Tamara and MOLENAAR, Remco J., 2020, 2D and 3D in vitro assays to quantify the invasive behavior of glioblastoma stem cells in response to SDF-1[alpha]. Biotechniques [online]. 2020. Vol. 69, no. 5, p. 339–346. [Accessed 10 April 2025]. DOI 10.2144/btn-2020-0046. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=19525
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Record is a part of a journal

Title:Biotechniques
Shortened title:BioTechniques
Publisher:Eaton Pub. Co.
ISSN:0736-6205
COBISS.SI-ID:27332096 New window

Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P1-0245-2019
Name:Ekotoksiologija, toksikološka genomika in karcinogeneza
Funder:ARIS - Slovenian Research and Innovation Agency
Project number:Z3-1870-2019
Name:Vpliv mezenhimskih matičnih celic na odpornost glioblastoma na terapijo
Funder:Other - Other funder or multiple funders
Funding programme:Dutch Cancer Society
Project number:UVA 2014-6839 and UVA 2016-10460
Funder:EC - European Commission
Funding programme:IVY Interreg Fellowship 2018
Funder:Other - Other funder or multiple funders
Funding programme:Fondation pour la Recherche Nuovo-Soldati 2019
Funder:EC - European Commission
Funding programme:European Program of Cross-Border Cooperation for Slovenia-Italy Interreg
Acronym:TRANS-GLIOMA
Funder:ARIS - Slovenian Research and Innovation Agency
Name:postdoctoral grant

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License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
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