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Title:Development and validation of a new TaqMan real-time PCR for detection of 'Candidatus phytoplasma pruni'
Authors:ID Kogej Zwitter, Zala (Author)
ID Dermastia, Marina (Author)
ID Mehle, Nataša (Author)
Files:URL URL - Source URL, visit https://www.mdpi.com/2076-0817/9/8/642
 
.pdf PDF - Presentation file, download (2,00 MB)
MD5: 276BC66C24180804B524113FCCD46039
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:Phytoplasmas of the 16SrIII group are wide spread, and have a broad plant host range. Among these, ‘Candidatus phytoplasma pruni’ (‘Ca. P. pruni’; phytoplasmas of 16SrIII subgroup A) can cause serious diseases in Prunus species and ‘Ca. P. pruni’-related strains can infect other plant species, including grapevines. In this study, a new real-time PCR detection system was developed for ‘Ca. P. pruni’ using TaqMan chemistry. This test was designed to detect ‘Ca. P. pruni’, by amplifying the species-specific secY gene. In addition, a test to amplify the group-specific 16S rRNA gene region was also developed. The performances of both tests were evaluated. The test that amplifies the secY gene provided reliable and quick detection of ‘Ca. P. pruni’. Using the newly developed and validated test, ‘Ca. P. pruni’ was not found in any of the 434 field samples collected from different plants species grown in different regions of Slovenia.
Keywords:phytoplasma, X-disease, real-time PCR, Prunus
Publication status:Published
Publication version:Version of Record
Publication date:07.08.2020
Year of publishing:2020
Number of pages:str. 1-13
Numbering:Vol. 9, iss. 8
PID:20.500.12556/DiRROS-19522 New window
UDC:632
ISSN on article:2076-0817
DOI:10.3390/pathogens9080642 New window
COBISS.SI-ID:24707587 New window
Note:Nasl. z nasl. zaslona; Opis vira z dne 10. 8. 2020;
Publication date in DiRROS:22.07.2024
Views:563
Downloads:321
Metadata:XML DC-XML DC-RDF
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KOGEJ ZWITTER, Zala, DERMASTIA, Marina and MEHLE, Nataša, 2020, Development and validation of a new TaqMan real-time PCR for detection of “Candidatus phytoplasma pruni.” Pathogens [online]. 2020. Vol. 9, no. 8, p. 1–13. [Accessed 10 April 2025]. DOI 10.3390/pathogens9080642. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=19522
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Record is a part of a journal

Title:Pathogens
Shortened title:Pathogens
Publisher:MDPI
ISSN:2076-0817
COBISS.SI-ID:523338009 New window

Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P4-0165-2015
Name:Biotehnologija in sistemska biologija rastlin

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Slovenian
Title:Development and validation of a new TaqMan real-time PCR for detection of Candidatus phytoplasma pruni
Keywords:rastline, fitoplazma, bolezni, PCR


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