| Naslov: | Polysaccharide-based bilayer coatings for biofilm-inhibiting surfaces of medical devices |
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| Avtorji: | ID Ajdnik, Urban (Avtor)
ID Luxbacher, Thomas (Avtor)
ID Vesel, Alenka (Avtor)
ID Štern, Alja (Avtor)
ID Žegura, Bojana (Avtor)
ID Trček, Janja (Avtor)
ID Fras Zemljič, Lidija (Avtor) |
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| Datoteke: |  URL - Izvorni URL, za dostop obiščite https://www.mdpi.com/1996-1944/14/16/4720
 PDF - Predstavitvena datoteka, prenos (6,81 MB)
MD5: 3185A94C8888F3821D4F3E9672E8EDE5
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  NIB - Nacionalni inštitut za biologijo
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| Povzetek: | Chitosan (Chi) and 77KS, a lysine-derived surfactant, form polyelectrolyte complexes that reverse their charge from positive to negative at higher 77KS concentrations, forming aggregates that have been embedded with amoxicillin (AMOX). Dispersion of this complex was used to coat polydimethylsiloxane (PDMS) films, with an additional layer of anionic and hydrophilic hyaluronic acid (HA) as an outer adsorbate layer to enhance protein repulsion in addition to antimicrobial activity by forming a highly hydrated layer in combination with steric hindrance. The formed polysaccharide-based bilayer on PDMS was analyzed by water contact angle measurements, X-ray photoelectron spectroscopy (XPS), and surface zeta (ζ)-potential. All measurements show the existence and adhesion of the two layers on the PDMS surface. Part of this study was devoted to understanding the underlying protein adsorption phenomena and identifying the mechanisms associated with biofouling. Thus, the adsorption of a mixed-protein solution (bovine serum albumin, fibrinogen, γ-globulin) on PDMS surfaces was studied to test the antifouling properties. The adsorption experiments were performed using a quartz crystal microbalance with dissipation monitoring (QCM-D) and showed improved antifouling properties by these polysaccharide-based bilayer coatings compared to a reference or for only one layer, i.e., the complex. This proves the benefit of a second hyaluronic acid layer. Microbiological and biocompatibility tests were also performed on real samples, i.e., silicone discs, showing the perspective of the prepared bilayer coating for medical devices such as prostheses, catheters (balloon angioplasty, intravascular), delivery systems (sheaths, implants), and stents. |
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| Ključne besede: | polysaccharides, chitosan, hyaluronic acid, anti-biofilm, silicone |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 21.08.2021 |
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| Leto izida: | 2021 |
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| Št. strani: | str. 1-22 |
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| Številčenje: | Vol. 14, iss. 16 |
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| PID: | 20.500.12556/DiRROS-19469  |
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| UDK: | 677.017:544.7 |
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| ISSN pri članku: | 1996-1944 |
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| DOI: | 10.3390/ma14164720 |
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| COBISS.SI-ID: | 73991171 |
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| Opomba: | Soavtorji: Thomas Luxbacher, Alenka Vesel, Alja Štern, Bojana Žegura, Janja Trček, Lidija Fras Zemljič;
Nasl. z nasl. zaslona;
Opis vira z dne 25. 8. 2021;
Št. članka 720;
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| Datum objave v DiRROS: | 19.07.2024 |
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| Število ogledov: | 481 |
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| Število prenosov: | 224 |
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| Metapodatki: |    |
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