| Title: | Studying cell death initiation using a digital microscope |
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| Authors: | ID Arnšek, Tina (Author)
ID Golob, Nuša (Author)
ID Marondini, Nastja (Author)
ID Pompe Novak, Maruša (Author)
ID Gruden, Kristina (Author)
ID Lukan, Tjaša (Author) |
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| Files: |  URL - Source URL, visit https://www.jove.com/t/65824/studying-cell-death-initiation-using-a-digital-microscope
 PDF - Presentation file, download (1,13 MB)
MD5: 13C0FBCA9F2F84C1EE478248732BCCC6
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | Hypersensitive response (HR)-conferred resistance is an effective defense response that can be determined by the N resistance genes. HR is manifested as the formation of cell death zones on inoculated leaves. Here, a protocol for studying the rate of cell death initiation by imaging inoculated leaves in the time between the cell death initiation and the cell death appearance using a digital microscope is presented. The digital microscope enables a continuous imaging process in desired intervals, which allows an accurate determination of cell death initiation rate up to minutes exactly, as opposed to hours in traditional methods. Imaging with the digital microscope is also independent of light and can therefore be used during day and night without disturbing the circadian rhythm of the plant. Different pathosystems resulting in programmed cell death development could be studied using this protocol with minor modifications. Overall, the protocol thus allows simple, accurate, and inexpensive identification of cell death initiation rate. |
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| Keywords: | digital microscope, cell death, inoculated leaves |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 10.11.2023 |
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| Year of publishing: | 2023 |
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| Number of pages: | str. 1-9 |
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| Numbering: | Vol. 201, [article no.] ǂ3027 |
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| PID: | 20.500.12556/DiRROS-19271  |
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| UDC: | 577.2 |
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| ISSN on article: | 1940-087X |
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| DOI: | 10.3791/65824 |
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| COBISS.SI-ID: | 177346819 |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 13. 12. 2023;
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| Publication date in DiRROS: | 12.07.2024 |
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| Views: | 344 |
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| Downloads: | 165 |
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