| Title: | Influence of alkylthio and arylthio derivatives of tert-butylquinone on the induction of DNA damage in a human hepatocellular carcinoma cell line (HepG2) |
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| Authors: | ID Djordjević, Jelena (Author)
ID Kolarević, Stoimir (Author)
ID Jovanović Marić, Jovana (Author)
ID Kračun-Kolarević, Margareta (Author)
ID Žegura, Bojana (Author)
ID Štern, Alja (Author)
ID Sladić, Dušan M. (Author)
ID Novaković, Irena (Author)
ID Vuković-Gačić, Branka (Author) |
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| Files: |  URL - Source URL, visit https://www.sciencedirect.com/science/article/pii/S0887233324001127?dgcid=coauthor
 DOCX - Presentation file, download (7,34 MB)
MD5: 9386A1E6839152BCD3A6C332D04DFE45
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | The aim of this study was to investigate the effects of tert-butylquinone (TBQ) and its alkylthio and arylthio
derivatives on DNA in vitro, using acellular and cellular test systems. Direct interaction with DNA was studied
using the plasmid pUC19. Cytotoxic (MTS assay) and genotoxic (comet assay and γH2AX focus assays) effects,
and their influence on the cell cycle were studied in the HepG2 cell line. Our results show that TBQ and its
derivatives did not directly interact with DNA. The strongest cytotoxic effect on the HepG2 cells was observed for
the derivative 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone (IC50 64.68 and 55.64 μM at 24-h and 48-h
treatment, respectively). The tested derivatives did not significantly influence the cell cycle distribution in the
exposed cellular populations. However, all derivatives showed a genotoxic activity stronger than that of TBQ in
the comet assay, with 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone producing the strongest effect. The
same derivative also induced DNA double-strand breaks in the γH2AX focus assay. |
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| Keywords: | TBQ derivatives, HepG2 cell line, comet assay, γH2AX assay, cell cycle analysis, cytotoxicity |
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| Publication status: | Published |
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| Publication version: | Preprint, working version of publication, not peer-reviewed |
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| Publication date: | 01.08.2024 |
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| Year of publishing: | 2024 |
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| Number of pages: | str. 1-8 |
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| Numbering: | Vol. 99, [article no.] ǂ105882 |
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| PID: | 20.500.12556/DiRROS-19232  |
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| UDC: | 577.2 |
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| ISSN on article: | 1879-3177 |
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| DOI: | 10.1016/j.tiv.2024.105882 |
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| COBISS.SI-ID: | 200413443 |
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| Note: | Soavtorji: Stoimir Kolarević, Jovana Jovanović Marić, Margareta Kračun-Kolarević, Bojana Žegura, Alja Štern, Dušan Sladić, Irena Novaković, Branka Vuković-Gačić;
Nasl. z nasl. zaslona;
Opis vira z dne 2. 7. 2024;
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| Publication date in DiRROS: | 11.07.2024 |
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| Views: | 378 |
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| Downloads: | 239 |
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