| Title: | Exploring the safety of cannabidiol (CBD) : a comprehensive in vitro evaluation of the genotoxic and mutagenic potential of a CBD isolate and extract from Cannabis sativa L |
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| Authors: | ID Štern, Alja (Author)
ID Novak, Matjaž (Author)
ID Kološa, Katja (Author)
ID Trontelj, Jurij (Author)
ID Žabkar, Sonja (Author)
ID Šentjurc, Tjaša (Author)
ID Filipič, Metka (Author)
ID Žegura, Bojana (Author) |
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| Files: |  URL - Source URL, visit https://www.sciencedirect.com/science/article/pii/S0753332224008539
 PDF - Presentation file, download (4,31 MB)
MD5: AFEB7FAEAEE10D01BBEF4F7273FE3144
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | Cannabidiol (CBD), a naturally occurring cyclic terpenoid found in Cannabis sativa L., is renowned for its diverse
pharmacological benefits. Marketed as a remedy for various health issues, CBD products are utilized by patients
as a supplementary therapy or post-treatment failure, as well as by healthy individuals seeking promised advantages. Despite its widespread use, information regarding potential adverse effects, especially genotoxic
properties, is limited. The present study is focused on the mutagenic and genotoxic activity of a CBD isolate
(99.4 % CBD content) and CBD-rich Cannabis sativa L extract (63.6 % CBD content) in vitro. Both CBD samples
were non-mutagenic, as determined by the AMES test (OECD 471) but exhibited cytotoxicity for HepG2 cells
(~IC50 (4 h) 26 µg/ml, ~IC50 (24 h) 6–8 µg/ml, MTT assay). Noncytotoxic concentrations induced upregulation of
genes encoding metabolic enzymes involved in CBD metabolism, and CBD oxidative as well as glucuronide
metabolites were found in cell culture media, demonstrating the ability of HepG2 cells to metabolize CBD. In this
study, the CBD samples were found non-genotoxic. No DNA damage was observed with the comet assay, and no
influence on genomic instability was observed with the cytokinesis block micronucleus and the γH2AX and p-H3
assays. Furthermore, no changes in the expression of genes involved in genotoxic stress response were detected in
the toxicogenomic analysis, after 4 and 24 h of exposure. Our comprehensive study contributes valuable insights
into CBD’s safety profile, paving the way for further exploration of CBD’s therapeutic applications and potential
adverse effects. |
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| Keywords: | cannabidiol, CBD, metabolism, cytotoxicity, genotoxicity, mutagenicity |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 01.08.2024 |
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| Year of publishing: | 2024 |
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| Number of pages: | str. 1-13 |
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| Numbering: | Vol. 177, [article no.] ǂ116969 |
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| PID: | 20.500.12556/DiRROS-19204  |
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| UDC: | 615.9 |
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| ISSN on article: | 1950-6007 |
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| DOI: | 10.1016/j.biopha.2024.116969 |
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| COBISS.SI-ID: | 200108803 |
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| Note: | Soavtorji: Matjaž Novak, Katja Kološa, Jurij Trontelj, Sonja Žabkar, Tjaša Šentjurc, Metka Filipič, Bojana Žegura;
Nasl. z nasl. zaslona;
Opis vira z dne 28. 6. 2024;
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| Publication date in DiRROS: | 09.07.2024 |
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| Views: | 291 |
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| Downloads: | 228 |
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