Digitalni repozitorij raziskovalnih organizacij Slovenije

Izpis gradiva
A+ | A- | Pomoč | SLO | ENG

Naslov:Genetic polymorphisms in homologous recombination repair genes in healthy Slovenian population and their influence on DNA damage
Avtorji:ID Goričar, Katja (Avtor)
ID Erčulj, Nina (Avtor)
ID Zadel, Maja (Avtor)
ID Dolžan, Vita (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (532,36 KB)
MD5: 73F89171D7E72B7985CABA592EE16B9C
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo OI - Onkološki inštitut Ljubljana
Povzetek:Background. Homologous recombination (HR) repair is an important mechanism involved in repairing double-strand breaks in DNA and for maintaining genomic stability. Polymorphisms in genes coding for enzymes involved in this pathway may influence the ca pa city for DNA repair. The aim of this study was to select tag single nucleotide polymorphisms (SNPs) in specific genes involved in HR repair, to determine their allele frequencies in a healthy Slovenian population and their influence on DNA damage detected with comet assay. Materials and methods. In total 373 individuals were genotyped for nine tag SNPs in three genes: XRCC3 722C>T, XRCC3 -316A>G, RAD51 -98G>C, RAD51 -61G>T, RAD51 1 522T>G, NBS1 553G>C, NBS1 1197A>G, NBS1 37117C>T and NBS1 3474A>C using competitive allele-specific amplification (KASPar assay). Comet assay was performed in a subgroup of 26 individuals to determine the influence of selected SNPs on DNA damage. Results. We observed that age significantly affected genotype frequencies distribution of XRCC3 -316A>G (P = 0.039) in healthy male blood donors. XRCC3 722C>T (P = 0.005), RAD51 -61G>T IP = 0.023) and NBS1 553G>C (P = 0.008) had a statistically significant influence on DNA damage. Conclusions. XRCC3 722C> T, RAD51 -61 G> T and NBS 1 553G>C polymorphisms significantly affect the repa ir of damaged DNA and may be of clinical importance as they are common in Slovenian population.
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:01.01.2012
Založnik:Association of Radiology and Oncology
Leto izida:2012
Št. strani:str. 46-53, IV
Številčenje:Vol. 46, no. 1
Izvor:Ljubljana
PID:20.500.12556/DiRROS-18528 Novo okno
UDK:575.111
ISSN pri članku:1318-2099
DOI:10.2478/v10019-012-0001-7 Novo okno
COBISS.SI-ID:29380057 Novo okno
Avtorske pravice:by Authors
Opomba:Soavtorji: Nina Erčulj, Maja Zadel, Vita Dolžan; BSDOCID162092;
Datum objave v DiRROS:22.03.2024
Število ogledov:529
Število prenosov:158
Metapodatki:XML DC-XML DC-RDF
:
Kopiraj citat
  
Objavi na:Bookmark and Share


Postavite miškin kazalec na naslov za izpis povzetka. Klik na naslov izpiše podrobnosti ali sproži prenos.

Gradivo je del revije

Naslov:Radiology and oncology
Skrajšan naslov:Radiol. oncol.
Založnik:Slovenian Medical Society - Section of Radiology, Croatian Medical Association - Croatian Society of Radiology
ISSN:1318-2099
COBISS.SI-ID:32649472 Novo okno

Sekundarni jezik

Jezik:Slovenski jezik
Naslov:Genetski polimorfizmi poti popravljanja napak na DNA s homologno rekombinacijo v zdravi slovenski populaciji in njihov vpliv na stopnjo poškodb DNA


Nazaj