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Naslov:Subchronic exposure of rats to sublethal dose of microcystin-YR induces DNA damage in multiple organs
Avtorji:ID Filipič, Metka (Avtor)
ID Žegura, Bojana (Avtor)
ID Sedmak, Bojan (Avtor)
ID Horvat-Žnidaršič, Irena (Avtor)
ID Milutinović Živin, Aleksandra (Avtor)
ID Šuput, Dušan (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (142,90 KB)
MD5: CAA6C3D171D0F77057A7F40C92824141
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo OI - Onkološki inštitut Ljubljana
Povzetek:Background. Microcystins (MCs) are cyclic heptapeptides that are considered tobe liver specific toxins. They are potent tumour promoters and recent studies indicate that they are also genotoxic. In this study we measured DNA damage in lymphocytes, liver, kidney (cortex and medulla), lung, spleen and brain cells of male Fisher F344 rats that were exposed to sublethal dose (every second day 10 Ugžkg b.w.č i.p) of microeysrin-YR (MCYR) for one month. Methods. At the end of exposure the animals were sacrificed, the lymphocytes were isolated from blood taken from jugular vein, liver cells were obtained byperfusion with collagenase A and the cells from other organs were isolated by incubating small tissue pieces with eollagenase A. The DNA damage in isolated cells was measured with the single cells gel electrophoresis (SCGE) also called the comet assay. Results. A significant increase of the % tail DNAin MCYR-exposed animals compared to the nonexposed control ones was observed in brain (2.5 fold), liver (2.1 fold), kidney medulla (1.9 fold), kidney cortex (1.8 fold) and lung (1.7 fold) cells, while the DNA from lymphocytes and spleen cells was not affected. Conclusion. This study demonstrated that subehronic exposure to sublethal doses of MCs can induce systemicgenotoxicity in mammals, and it affects not only the liver but also other vital organs.
Ključne besede:DNA damage, comet assay, cyanobacteria, bacterial toxins, rats, inbred F344
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:01.01.2007
Založnik:Association of Radiology and Oncology
Leto izida:2007
Št. strani:str. 15-22
Številčenje:no. 1, Vol. 41
Izvor:Ljubljana
PID:20.500.12556/DiRROS-18219 Novo okno
UDK:616
ISSN pri članku:1318-2099
COBISS.SI-ID:1724751 Novo okno
Avtorske pravice:by Authors
Datum objave v DiRROS:20.02.2024
Število ogledov:792
Število prenosov:178
Metapodatki:XML DC-XML DC-RDF
:
FILIPIČ, Metka, ŽEGURA, Bojana, SEDMAK, Bojan, HORVAT-ŽNIDARŠIČ, Irena, MILUTINOVIĆ ŽIVIN, Aleksandra in ŠUPUT, Dušan, 2007, Subchronic exposure of rats to sublethal dose of microcystin-YR induces DNA damage in multiple organs. Radiology and oncology [na spletu]. 2007. Vol. 1, no. 41, p. 15–22. [Dostopano 8 april 2025]. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=18219
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Gradivo je del revije

Naslov:Radiology and oncology
Skrajšan naslov:Radiol. oncol.
Založnik:Slovenian Medical Society - Section of Radiology, Croatian Medical Association - Croatian Society of Radiology
ISSN:1318-2099
COBISS.SI-ID:32649472 Novo okno

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